Kovalenko Anton, Eliseeva Svetlana V, Collet Guillaume, El Abdellaoui Saïda, Natkunarajah Sharuja, Lerondel Stéphanie, Guénée Laure, Besnard Céline, Petoud Stéphane
Centre de Biophysique Moléculaire, CNRS UPR4301, Université d'Orléans, rue Charles Sadron, 45071 Orléans Cedex 2, France.
Le Studium Loire Valley Institute for Advanced Studies, 1 rue Dupanloup, 45000 Orléans, France.
J Am Chem Soc. 2024 May 15;146(19):12913-12918. doi: 10.1021/jacs.4c03406. Epub 2024 May 3.
Near-infrared (NIR) luminescence and photoacoustic (PA) imaging have attracted increasing attention for the real-time monitoring of biological samples due to high sensitivity, resolution, and pronounced signal detection depth, respectively. For improved contrast, both techniques require imaging agents possessing high absorption in the red-NIR range. Herein, we took advantage of a ternary complex formed with the anionic ytterbium(III) (2-thenoyltrifluoroacetonate) ([Yb(tta)]) and the cationic NIR-absorbing chromophore, 1,1'-diethyl-2,2'-dicarbocyanine (Cy), to evaluate its potential to act as a dual-mode NIR luminescence and PA imaging agent. We demonstrated that, upon excitation with red-NIR light, Cy[Yb(tta)] encapsulated into polystyrene nanoparticles is able to generate both NIR Yb emission and a PA signal in an imaging experiment performed in a tissue-mimicking phantom.
近红外（NIR）发光成像和光声（PA）成像分别因其高灵敏度、高分辨率和显著的信号检测深度，在生物样品的实时监测方面受到越来越多的关注。为了提高对比度，这两种技术都需要在红-近红外范围内具有高吸收的成像剂。在此，我们利用由阴离子镱（III）（2-噻吩甲酰三氟丙酮）（[Yb(tta)]）和阳离子近红外吸收发色团1,1'-二乙基-2,2'-二碳菁（Cy）形成的三元配合物，评估其作为双模式近红外发光和光声成像剂的潜力。我们证明，在用红-近红外光激发后，封装在聚苯乙烯纳米颗粒中的Cy[Yb(tta)]在组织模拟体模中进行的成像实验中能够产生近红外Yb发射和光声信号。
