Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese 905 Academy of Sciences, Xiamen 361021, 906, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese 905 Academy of Sciences, Xiamen 361021, 906, PR China.
Environ Int. 2024 May;187:108713. doi: 10.1016/j.envint.2024.108713. Epub 2024 May 1.
Nanoplastics (NPs) are increasingly pervasive in the environment, raising concerns about their potential health implications, particularly within aquatic ecosystems. This study investigated the impact of polystyrene nanoparticles (PSN) on zebrafish liver metabolism using liquid chromatography hybrid quadrupole time of flight mass spectrometry (LC-QTOF-MS) based non-targeted metabolomics. Zebrafish were exposed to 50 nm PSN for 28 days at low (L-PSN) and high (H-PSN) concentrations (0.1 and 10 mg/L, respectively) via water. The results revealed significant alterations in key metabolic pathways in low and high exposure groups. The liver metabolites showed different metabolic responses with L-PSN and H-PSN. A total of 2078 metabolite features were identified from the raw data obtained in both positive and negative ion modes, with 190 metabolites deemed statistically significant in both L-PSN and H-PSN groups. Disruptions in lipid metabolism, inflammation, oxidative stress, DNA damage, and amino acid synthesis were identified. Notably, L-PSN exposure induced changes in DNA building blocks, membrane-associated biomarkers, and immune-related metabolites, while H-PSN exposure was associated with oxidative stress, altered antioxidant metabolites, and liver injury. For the first time, L-PSN was found depolymerized in the liver by cytochrome P450 enzymes. Utilizing an analytical approach to the adverse outcome pathway (AOP), impaired lipid metabolism and oxidative stress have been identified as potentially conserved key events (KEs) associated with PSN exposure. These KEs further induced liver inflammation, steatosis, and fibrosis at the tissue and organ level. Ultimately, this could significantly impact biological health. The study highlights the PSN-induced effects on zebrafish liver metabolism, emphasizing the need for a better understanding of the risks associated with NPs contamination in aquatic ecosystems.
纳米塑料（NPs）在环境中日益普遍，引起了人们对其潜在健康影响的关注，尤其是在水生生态系统中。本研究使用基于液相色谱-四极杆飞行时间质谱联用（LC-QTOF-MS）的非靶向代谢组学方法，研究了聚苯乙烯纳米颗粒（PSN）对斑马鱼肝脏代谢的影响。斑马鱼通过水暴露于 50nm PSN 低（L-PSN）和高（H-PSN）浓度（分别为 0.1 和 10mg/L）28 天。结果表明，低和高暴露组关键代谢途径发生显著改变。L-PSN 和 H-PSN 组的肝脏代谢物表现出不同的代谢反应。从正、负离子模式下获得的原始数据中鉴定出 2078 种代谢物特征，在 L-PSN 和 H-PSN 组中均有 190 种代谢物具有统计学意义。结果发现脂质代谢、炎症、氧化应激、DNA 损伤和氨基酸合成受到干扰。值得注意的是，L-PSN 暴露导致 DNA 构建块、膜相关生物标志物和免疫相关代谢物发生变化，而 H-PSN 暴露与氧化应激、抗氧化代谢物改变和肝损伤有关。首次发现 L-PSN 在肝脏中被细胞色素 P450 酶解聚。利用一种分析方法对不良结局途径（AOP）进行分析，发现脂质代谢受损和氧化应激是与 PSN 暴露相关的潜在保守关键事件（KEs）。这些 KEs 进一步导致组织和器官水平的肝脏炎症、脂肪变性和纤维化。最终，这可能会对生物健康产生重大影响。该研究强调了 PSN 对斑马鱼肝脏代谢的影响，强调需要更好地了解 NPs 污染对水生生态系统的风险。
