Rivas M, Cordal de Bobbi M E, Raffo Palma M S, Moro A A
Instituto Nacional de Farmacología y Bromatología, Buenos Aires, Argentina.
Rev Argent Microbiol. 1985;17(2):69-73.
A total of 118 Staphylococcus aureus strains isolated from routine sampling, samples from food poisoning outbreaks and human clinical specimens were examined for the production of enterotoxins A, B, C, D and E. The toxic properties of strains were compared with other biochemical characteristics and with the sensitivity to antibiotics. Of the total strains examined, 17.8% (21 strains) produced enterotoxins, and of the toxigenic strains, 81% (17 strains) produced just one type of enterotoxin and 19% (4 strains) two types. Enterotoxin A production was found in 52.4% strains, the other enterotoxins detected in decreasing order of frequency were: C; B; AD; D; AB and BD. All the strains examined produced catalases, coagulases, thermonuclease and fermented glucose; 81 and 89.7% for toxigenic and non-toxigenic strains, respectively, fermented mannitol; 47.6 and 54.6% hydrolyzed casein and 47.6 and 52.6% gelatin; 85.7 and 92.8% produced yellow or orange pigment. Mixed acid fermentation was carried out in 100% and in 96.9%; acetoin was produced by 57.1 and 47.4%; one or more hemolysins were released by 85.7 and 92.8% of the toxigenic and non-toxigenic strains, respectively. Sensitivity to antibiotics was widespread among all the strains. No relation was found between enterotoxin B production and methicillin and tetracycline resistance. Neither the biochemical properties nor the sensitivity to antibiotics has been shown to correlate reliably with toxin production.
对从常规采样、食物中毒暴发样本和人类临床标本中分离出的118株金黄色葡萄球菌进行了肠毒素A、B、C、D和E产生情况的检测。将菌株的毒性特性与其他生化特征以及对抗生素的敏感性进行了比较。在所检测的全部菌株中，17.8%（21株）产生肠毒素，在产毒菌株中，81%（17株）仅产生一种类型的肠毒素，19%（4株）产生两种类型。发现52.4%的菌株产生肠毒素A，检测到的其他肠毒素按频率递减顺序为：C；B；AD；D；AB和BD。所有检测的菌株均产生过氧化氢酶、凝固酶、耐热核酸酶并发酵葡萄糖；产毒菌株和非产毒菌株中分别有81%和89.7%发酵甘露醇；47.6%和54.6%水解酪蛋白，47.6%和52.6%水解明胶；85.7%和92.8%产生黄色或橙色色素。100%和96.9%进行混合酸发酵；57.1%和47.4%产生乙酰甲基甲醇；产毒菌株和非产毒菌株中分别有85.7%和92.8%释放一种或多种溶血素。所有菌株对抗生素普遍敏感。未发现肠毒素B产生与对甲氧西林和四环素耐药之间存在关联。未显示生化特性或对抗生素的敏感性与毒素产生有可靠的相关性。
