Donner B, Helmboldt-Caesar U, Rensing L
Biology Department, University of Bremen, FRG.
Chronobiol Int. 1985;2(1):1-9. doi: 10.3109/07420528509055536.
Protein synthesis of Gonyaulax polyedra was analyzed by means of electron microscopic autoradiographs under constant conditions at different times of the 24-hr cycle. Circadian rhythmic changes in the synthesis rate of total protein were determined in the cytoplasm and chloroplasts of growing cells. Three independent series of experiments in constant light showed a maximum of grains per unit area during the 'subjective' dark phase (= phase that corresponds to the dark phase during a 12:12 hr LD cycle) in both compartments. Minimum and maximum grain number are different by a factor of 5-10. The maximum of total protein synthesis coincided with the maximum phase shift by cycloheximide pulses (1) suggesting protein species within the total pool involved in the mechanism of the circadian clock. A similar rhythm of lower amplitude was observed in the mitochondria, but this rhythm cannot with certainty be attributed to these organelles. In a slowly growing culture a rhythm of total protein synthesis was observed that showed a smaller amplitude and a different phasing.
在24小时周期的不同时间，在恒定条件下通过电子显微镜放射自显影片分析了多边膝沟藻的蛋白质合成。测定了生长细胞的细胞质和叶绿体中总蛋白质合成速率的昼夜节律变化。在连续光照下进行的三个独立系列实验表明，在两个区室中，在“主观”黑暗期（=对应于12:12小时光照/黑暗周期中的黑暗期的阶段）单位面积的颗粒数最多。最小和最大颗粒数相差5至10倍。总蛋白质合成的最大值与环己酰亚胺脉冲引起的最大相移相一致(1)，这表明总库中的蛋白质种类参与了昼夜节律钟的机制。在线粒体中观察到类似的较低幅度的节律，但这种节律不能确定归因于这些细胞器。在缓慢生长的培养物中观察到总蛋白质合成的节律，其幅度较小且相位不同。
