Taylor W R, Dunlap J C, Hastings J W
J Exp Biol. 1982 Apr;97:121-36. doi: 10.1242/jeb.97.1.121.
One-hour pulses of anisomycin (0.3 microM), streptimidone (30 microM) and cycloheximide (5 microM) caused strong phase-shifts (either advances or delays, of up to 12 h) in the circadian rhythm of the bioluminescence glow in the marine photosynthetic dinoflagellate, Gonyaulax polyedra. Similar pulses of emetine (0.1-100 microM) caused small (less than 4 h) phase shifts. Drug pulses have quantitatively different effects when applied at different phases of the circadian cycle, thus giving rise to 'phase response curves' (PRC's). The results lend additional support to the generalization, based on results from several different organisms, that 80s ribosome protein synthesizing system is of key importance in the mechanism responsible for circadian rhythms.
用茴香霉素（0.3微摩尔）、链霉素（30微摩尔）和环己酰亚胺（5微摩尔）进行一小时的脉冲处理，会使海洋光合鞭毛藻多甲藻（Gonyaulax polyedra）生物发光的昼夜节律产生强烈的相位偏移（提前或延迟，最多可达12小时）。类似的依米丁（0.1 - 100微摩尔）脉冲处理会引起较小（小于4小时）的相位偏移。当在昼夜周期的不同阶段施加药物脉冲时，其效果在数量上有所不同，从而产生“相位响应曲线”（PRC）。基于来自几种不同生物体的结果得出的普遍观点认为，80S核糖体蛋白质合成系统在昼夜节律的机制中至关重要，这些结果为这一观点提供了更多支持。
