Hekkens W T, van Rijn P A, Lukac-Bajalo J, Ungerer R, Rietveld W J
Department of Physiology and Physiological Physics, University of Leiden, The Netherlands.
Chronobiol Int. 1985;2(2):103-8. doi: 10.3109/07420528509055548.
Circadian changes in activities and electrophoretic pattern of the intestinal brushborder enzymes alkaline phosphatase (EC 3.1.3.1), isomaltase (EC 3.2.1.10) and sucrase (EC 3.2.1.26) of normal rats and of rats with a lesion of the dorsomedial hypothalamus (DMH-rats) were studied. In contrast to the rhythm found in normal rats, there are no significant differences between the average enzyme activities in the light or dark period for the DMH-rats. The peak ratio in the electrophoretic pattern for sucrase-isomaltase (SI) over pro-sucrase-isomaltase (pro-SI) changes over 24 hr with higher values during the active (dark) period of the normal rat. In DMH-rats this ratio is much higher, but no significant difference could be demonstrated between the values near the acrophase in these rats.
研究了正常大鼠和下丘脑背内侧损伤大鼠（DMH大鼠）肠道刷状缘酶碱性磷酸酶（EC 3.1.3.1）、异麦芽糖酶（EC 3.2.1.10）和蔗糖酶（EC 3.2.1.26）活性及电泳图谱的昼夜变化。与正常大鼠的节律不同，DMH大鼠在光照期或黑暗期的平均酶活性之间没有显著差异。正常大鼠在活动期（黑暗期）蔗糖酶-异麦芽糖酶（SI）与前蔗糖酶-异麦芽糖酶（pro-SI）电泳图谱的峰值比在24小时内变化，且在活动期（黑暗期）值更高。在DMH大鼠中，该比值更高，但在这些大鼠的高峰期附近的值之间未显示出显著差异。
