Sebastio G, Hunziker W, Ballabio A, Auricchio S, Semenza G
FEBS Lett. 1986 Nov 24;208(2):460-4. doi: 10.1016/0014-5793(86)81069-9.
We have compared the appearance in the small intestine of baby rabbits, of sucrase and isomaltase activities and of the sucrase-isomaltase mRNA. For the latter we used a cDNA probe encompassing approximately 4.1 kb from the 5'-end of pro-sucrase-isomaltase cDNA [(1986) Cell 46, 227-234]. Sucrase-isomaltase mRNA and the enzyme activities developed simultaneously from the 15th day after birth onwards. Over two orders of magnitude the enzymatic activities and sucrase-isomaltase mRNA matched one another closely, thus ruling out translation as the main site of biosynthetic control during spontaneous development, while rendering very probable transcription as the primary site of control. However, we cannot rule out the possibility that, prior to day 15, sucrase-isomaltase mRNA might be degraded so rapidly that it is not translated.
我们比较了幼兔小肠中蔗糖酶和异麦芽糖酶的活性以及蔗糖酶-异麦芽糖酶mRNA的表现。对于后者，我们使用了一个cDNA探针，该探针包含来自蔗糖酶-异麦芽糖酶原cDNA 5'端约4.1 kb的片段[（1986年）《细胞》46卷，227 - 234页]。从出生后第15天起，蔗糖酶-异麦芽糖酶mRNA和酶活性同时出现。在两个数量级以上，酶活性和蔗糖酶-异麦芽糖酶mRNA彼此紧密匹配，因此排除了翻译是自发发育过程中生物合成控制的主要位点的可能性，同时使转录很可能成为控制的主要位点。然而，我们不能排除在第15天之前蔗糖酶-异麦芽糖酶mRNA可能降解得如此之快以至于无法被翻译的可能性。
