Department of Gynecologic Oncology, Basaksehir Cam ve Sakura City Hospital, Basaksehir, Turkey.
Eur Rev Med Pharmacol Sci. 2024 Apr;28(8):3048-3055. doi: 10.26355/eurrev_202404_36020.
Despite the multiple available treatment modalities, cervical cancer is one of the leading causes of mortality and morbidity among female gynecological cancers. Endoplasmic Reticulum (ER) is an effective organelle in ensuring cell homeostasis and is closely related to the development of cancer. Esculetin is a coumarin derivative that has anticancer, anti-inflammatory, antioxidant, and neuroprotective effects. Esculetin may have an anticancer effect by inducting apoptosis and ER stress. In this study, we evaluate that esculetin has an anti-tumor effect on human cervical cancer-derived (HeLa) cells via ER stress.
Esculetin was applied to the HeLa cells, and a viability test was performed using the methyl thiazolyl tetrazolium proliferation (MTT) assay. Expression levels of apoptotic genes and anti-apoptotic genes were determined by real-time polymerase chain reaction. The results were statistically evaluated.
Analysis of the MTT assay detected that esculetin inhibited HeLa cell viability development. Based on Western blot and quantitative real-time polymerase chain reaction (qPCR) analyses, esculetin destroyed cervical cancer cells via the ER stress pathway.
The results showed that esculetin may have a potent antitumoral effect. It can potentially be utilized in the pharmacological therapy of cervical cancer.
尽管有多种治疗方法，但宫颈癌仍是女性妇科癌症死亡和发病的主要原因之一。内质网（ER）是确保细胞内环境稳定的有效细胞器，与癌症的发展密切相关。秦皮素是一种香豆素衍生物，具有抗癌、抗炎、抗氧化和神经保护作用。秦皮素可能通过诱导细胞凋亡和内质网应激发挥抗癌作用。在这项研究中，我们通过内质网应激评估秦皮素对人宫颈癌（HeLa）细胞的抗肿瘤作用。
将秦皮素应用于 HeLa 细胞，并通过甲基噻唑基四唑增殖（MTT）测定法进行细胞活力测试。通过实时聚合酶链反应（PCR）测定法确定凋亡基因和抗凋亡基因的表达水平。对结果进行了统计学评估。
MTT 分析检测到秦皮素抑制了 HeLa 细胞活力的发展。基于 Western blot 和定量实时 PCR（qPCR）分析，秦皮素通过内质网应激途径破坏了宫颈癌细胞。
结果表明，秦皮素可能具有强大的抗肿瘤作用。它可能被用于宫颈癌的药物治疗。
