Applied Biology Division, CSIR-Indian Institute of Chemical Technology, Hyderabad, Telangana, India.
Applied Biology Division, CSIR-Indian Institute of Chemical Technology, Hyderabad, Telangana, India; Department of Genetics, Osmania University, Hyderabad, Telangana, India.
Gene. 2021 Mar 1;771:145370. doi: 10.1016/j.gene.2020.145370. Epub 2020 Dec 24.
The global burden of cervical cancer from low and middle-income groups is increasing at alarming rates with more than half a million women being diagnosed every year. Although the disease is largely preventable when screened and diagnosed in earlier stages, the development of resistance and relapse had resulted in a poor prognosis. Therefore, a comprehensive approach needs to be put forward to understand and develop new preventive and therapeutic strategies to effectively combat cancer. Recently, much attention has been diverted to plant-derivatives for the treatment as they exhibit potent anti-cancer properties and side-effects caused by chemotherapeutic agents can also be prevented. Oleanolic acid and Esculetin are natural compounds known for their anti-cancer properties. Hence, the present study investigates the effect and mechanism of these compounds on cervical carcinoma, using HeLa cells. Posttreatment, it was observed that these compounds inhibited proliferation by both arresting the cells in the sub G1 phase and inducing senescence. Also, a marked reduction in the migration and cell survival was observed, as evidenced by results obtained from wound healing assay and Annexin V-FITC/PI staining. Furthermore, studies on the expression pattern of genes involved in major signaling pathways demonstrated a profound effect of these compounds. Taken together, the results of our study suggest that both Oleanolic acid and esculetin serve as a plausible therapeutic agent.
来自中低收入群体的宫颈癌全球负担正以惊人的速度增长，每年有超过 50 万名女性被诊断出患有该病。虽然通过筛查和早期诊断可以在很大程度上预防这种疾病，但耐药性和复发的发展导致了预后不良。因此，需要提出一种综合方法来了解和开发新的预防和治疗策略，以有效对抗癌症。最近，人们对植物衍生药物的治疗作用给予了极大的关注，因为它们具有很强的抗癌特性，同时也可以预防化疗药物引起的副作用。齐墩果酸和秦皮素是具有抗癌特性的天然化合物。因此，本研究使用 HeLa 细胞，研究了这些化合物对宫颈癌的作用及其机制。治疗后，观察到这些化合物通过将细胞阻滞在 sub G1 期并诱导衰老来抑制增殖。另外，划痕愈合试验和 Annexin V-FITC/PI 染色的结果表明，细胞迁移和存活能力显著降低。此外，对涉及主要信号通路的基因表达模式的研究表明，这些化合物具有深远的影响。综上所述，本研究结果表明，齐墩果酸和秦皮素均可作为合理的治疗药物。
