Laboratorio de Investigación en Nutrición, Facultad de Medicina, Universidad Autónoma del Estado de México, Paseo Tollocan esquina Jesús Carranza S/N, Colonia Moderna de la Cruz, Toluca, México.
Eur Rev Med Pharmacol Sci. 2024 Apr;28(8):3275-3286. doi: 10.26355/eurrev_202404_36056.
OBJECTIVE: This study aimed to compare two routes of administration and different dosages of streptozotocin (STZ) for the pharmacological induction of gestational diabetes mellitus (GDM) in pregnant CD1 females. MATERIALS AND METHODS: 35 female CD1 mice were divided into 5 groups (n = 7). Diabetes mellitus (DM) was induced with STZ by two routes and two doses: 1) Control Group without administration of STZ (CL), 2) Intraperitoneal Group with 200 mg of STZ/Kg of weight (IP200), 3) Intraperitoneal Group with 230 mg of STZ/Kg of weight (IP230), 4) Subcutaneous Group with 200 mg of STZ/Kg of weight (SC200) and 5) Subcutaneous Group with 230 mg of STZ/Kg of weight (SC230). Body weight, food and water intake, glycemia, Homeostatic Model Assessment of Insulin Resistance Index (HOMA-IR), survival, and birth rate were identified. RESULTS: The SC230 group turned out to be the most effective dose and route for the induction of GDM in pregnant females. This scheme managed to reproduce sustained hyperglycemia with high HOMA-IR, the presence of polyphagia, polydipsia, and weight loss. In addition, the birth rate and survival were high compared to the other doses and routes of administration. CONCLUSIONS: The administration of a single dose of 230 mg/kg of weight by subcutaneous route supposes advantages compared to previously used models since it decreases the physiological stress due to manipulation and the costs since it does not require repeated doses or adjuvants such as high lipid diets to potentiate the diabetogenic effect of STZ.
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目的：本研究旨在比较链脲佐菌素（STZ）两种给药途径和不同剂量用于诱导 CD1 雌性妊娠糖尿病（GDM）的药效学。材料与方法：35 只雌性 CD1 小鼠分为 5 组（n = 7）。用 STZ 经两种途径和两种剂量诱导糖尿病：1）未给予 STZ 的对照组（CL），2）200mg/kg 体重腹腔注射组（IP200），3）230mg/kg 体重腹腔注射组（IP230），4）200mg/kg 体重皮下注射组（SC200），5）230mg/kg 体重皮下注射组（SC230）。记录体重、食物和水的摄入、血糖、稳态模型评估的胰岛素抵抗指数（HOMA-IR）、存活率和出生率。结果：SC230 组是诱导妊娠雌鼠 GDM 最有效的剂量和途径。该方案成功复制了持续高血糖伴高 HOMA-IR、多食、多饮和体重减轻。此外，与其他剂量和给药途径相比，出生率和存活率较高。结论：与之前使用的模型相比，通过皮下途径单次给予 230mg/kg 体重的 STZ 具有优势，因为它减少了操作引起的生理应激和成本，并且不需要重复剂量或佐剂（如高脂饮食）来增强 STZ 的致糖尿病作用。
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