Trivedi Anjali, Bhati Shivani, Joshi Aruna
Department of Botany, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara, India.
Nat Prod Res. 2024 May 6:1-8. doi: 10.1080/14786419.2024.2349807.
(L.) belonging to family Fabaceae is an economically important medicinal plant which isutilised in Dashmoolarishta. Various bioactive compounds have been isolated from whole plant and roots, and one of them is an important phenolic compound - caffeic acid (CA). This phenolic acid and its derivatives have antioxidant, anti-inflammatory, anticarcinogenic and hepatocarcinoma, a highly aggressive and causing considerable mortality across the world. In the present study, leaf explants were placed on MS medium fortified with different concentration of cytokinin (BA/Kn) and auxin (IAA/NAA) for establishing callus cultures. MS medium fortified with BA (20 µM) and IAA (2 µM) was optimised for the same. Methanolic extracts of leaf sample (DG1) and sample (leaf derived callus) (DG2) were assessed for CA quantification using HPTLC. Thus, the chemical fingerprint that was obtained, confirmed that DG 2 of exhibited the potency to synthesise more amount of CA (316 ± 7.5 µg/g DW) in comparison to DG1 which was 194 ± 2.3 µg/g DW.
（豆科植物）是一种具有重要经济价值的药用植物，被用于制备十味药汁。已从其全株和根部分离出多种生物活性化合物，其中一种是重要的酚类化合物——咖啡酸（CA）。这种酚酸及其衍生物具有抗氧化、抗炎、抗癌和抗肝癌作用，肝癌是一种极具侵袭性且在全球导致相当高死亡率的疾病。在本研究中，将叶片外植体置于添加不同浓度细胞分裂素（BA/Kn）和生长素（IAA/NAA）的MS培养基上以建立愈伤组织培养。为此优化了添加BA（20µM）和IAA（2µM）的MS培养基。使用高效薄层色谱法（HPTLC）对叶片样品（DG1）和样品（叶片来源的愈伤组织）（DG2）的甲醇提取物进行咖啡酸定量分析。因此，所获得的化学指纹图谱证实，与DG1（194±2.3µg/g干重）相比，愈伤组织（DG2）具有合成更多量咖啡酸（316±7.5µg/g干重）的能力。
