Dar Shabeer Ahmad, Nawchoo Irshad Ahmad, Tyub Sumira, Kamili Azra Nahaid
Plant Tissue Culture Laboratory, Centre of Research for Development University of Kashmir, Srinagar, J&K, 190006 India.
Department of Botany university of Kashmir Srinagar, Jammu and Kashmir, 190006 India.
Biotechnol Rep (Amst). 2021 Nov 14;32:e00688. doi: 10.1016/j.btre.2021.e00688. eCollection 2021 Dec.
, an important medicinal plant belonging to family Solanaceae is under tremendous threat of extinction in its natural habitat due to the overexploitation by pharmaceutical industries. Present study is an attempt of establishing callus cultures of this important medicinal plant as callus has considerable potential as an alternative for production of secondary metabolites for industrial use, hence reducing pressure on natural populations. Callus cultures were established from leaf and root explants of . Murashige and Skoog (MS) media containing different concentration and combinations of 6-Benzyl Amino Purine (BAP), Naphthalene Acetic Acid (NAA), Kinetin (Kn) and 2,4- Dichloropheoxyacetic acid (2,4- D) were used for callus induction. Different phytohormonal combinations resulted in different types and degrees of callus. The combination of BAP and NAA on MS media supplemented with 0.5 mg/l BAP in combination with 1.0 mg/l NAA, was found to be the most efficient for in vitro callus development from root explants and from leaf explants most effective combination and concentration was 1 mg/l of both BAP and NAA. The maximum mean fresh weight of callus formed using root explants was 33.13 mg per explant and maximum fresh weight obtained from leaf explants was 22.14 mg per explants.
一种属于茄科的重要药用植物，由于制药行业的过度开发，其在自然栖息地正面临着巨大的灭绝威胁。目前的研究试图建立这种重要药用植物的愈伤组织培养，因为愈伤组织作为工业用次生代谢产物生产的替代物具有相当大的潜力，从而减轻对自然种群的压力。愈伤组织培养是从该植物的叶片和根外植体建立的。使用含有不同浓度和组合的6-苄基氨基嘌呤（BAP）、萘乙酸（NAA）、激动素（Kn）和2,4-二氯苯氧乙酸（2,4-D）的Murashige和Skoog（MS）培养基进行愈伤组织诱导。不同的植物激素组合导致了不同类型和程度的愈伤组织。在添加0.5mg/l BAP与1.0mg/l NAA组合的MS培养基上，BAP和NAA的组合被发现对根外植体的体外愈伤组织发育最有效，而对于叶片外植体，最有效的组合和浓度是BAP和NAA均为1mg/l。使用根外植体形成的愈伤组织的最大平均鲜重为每个外植体33.13mg，从叶片外植体获得的最大鲜重为每个外植体22.14mg。
