Key Laboratory of Fishery Drug Development of Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Aquatic Animal Immunology and Sustainable Aquaculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China.
Germany Reference Laboratory for KHVD, Institute of Infectology, Friedrich-Loffler-Institut (FLI), Federal Research Institute for Animal Health, Greifswald-Insel Riems, Germany.
J Fish Dis. 2024 Aug;47(8):e13960. doi: 10.1111/jfd.13960. Epub 2024 May 6.
In this issue, we established rapid, cost-effective, and simple detection methods including recombines polymerase amplification with lateral flow dipstick (RPA-LFD) and real-time RPA for cyprinid herpesvirus 3(CyHV-3), and evaluated their sensitivity, specificity, and applicability, the real-time RPA method could achieve sensitive diagnosis of CyHV-3 within 1.3 copies per reaction, respectively. The real-time RPA method is 10-fold more sensitive than RPA-LFD method. The exact number of CyHV-3 can be calculated in each sample by real-time RPA. The sera from koi also can be tested in these methods. In addition, no cross-reaction was observed with other related pathogens, including carp oedema virus (CEV), spring viraemia of carp virus (SVCV), cyprinid herpesvirus 1(CyHV-1), cyprinid herpesvirus 2(CyHV-2), type I grass carp reovirus (GCRV-I), type II GCRV (GCRV-II), type III GCRV (GCRV-III), and Aeromonas hydrophila.
在本期中，我们建立了快速、经济且简便的检测方法，包括重组聚合酶扩增与侧向流试纸条（RPA-LFD）和实时 RPA 检测鲤疱疹病毒 3（CyHV-3），并评估了它们的灵敏度、特异性和适用性，实时 RPA 方法可在每个反应中达到 1.3 拷贝的敏感诊断 CyHV-3。实时 RPA 方法比 RPA-LFD 方法灵敏 10 倍。实时 RPA 可计算每个样本中 CyHV-3 的准确数量。这些方法也可检测锦鲤的血清。此外，与其他相关病原体（包括鲤鱼水肿病毒（CEV）、草鱼出血病病毒（SVCV）、鲤疱疹病毒 1（CyHV-1）、鲤疱疹病毒 2（CyHV-2）、草鱼呼肠孤病毒 I 型（GCRV-I）、草鱼呼肠孤病毒 II 型（GCRV-II）、草鱼呼肠孤病毒 III 型（GCRV-III）和嗜水气单胞菌）无交叉反应。
