Paz Maria Gabriela Oliveira da, Bezerra Matheus Filgueira, Mendes Andrei Félix, da Silva Adalucia, Lima Gustavo Barbosa de, Gil Laura Helena Vega Gonzales, Pena Lindomar José, Diniz George Tadeu Nunes, Drexler Jan Felix, Oriá Reinaldo Barreto, Ferreira de Oliveira-Filho Edmilson, Reis Christian Robson de Souza
Department of Microbiology-Aggeu Magalhães Institute, Oswaldo Cruz Foundation, Recife, Brazil.
Department of Virology, Aggeu Magalhães Institute, Oswaldo Cruz Foundation, Recife, Brazil.
Foodborne Pathog Dis. 2024 Aug;21(8):517-520. doi: 10.1089/fpd.2023.0179. Epub 2024 May 6.
Hepatitis E virus (HEV) infects roughly 20 million people worldwide, causing self-limiting acute hepatic disease that can evolve into a chronic course. HEV-3, HEV-4, and HEV-7 genotypes are zoonotic and transmitted to humans by consuming raw or undercooked meat. Here, we developed an indirect ELISA based on the recombinant HEV-3 capsid and performed a seroprevalence study on domestic swine in northeastern Brazil. Our in-house ELISA was initially validated using a subset of 79 sera characterized by concordant results for two distinct commercial ELISA kits. Our ELISA exhibited excellent sensitivity (94%) and specificity (100%), with an area under the curve of 0.99 Further testing, including 212 swine sera, revealed a seroprevalence of 57.5% (95% confidence interval, 50.6-64.3%). Our findings indicate that the novel ELISA test could accurately detect specific anti-HEV antibodies in domestic pigs and should be further validated in humans and other mammals.
戊型肝炎病毒（HEV）在全球约感染2000万人，可引起自限性急性肝病，且可能发展为慢性病程。HEV-3、HEV-4和HEV-7基因型为人畜共患病原体，通过食用生肉或未煮熟的肉类传播给人类。在此，我们基于重组HEV-3衣壳开发了一种间接ELISA，并对巴西东北部的家猪进行了血清流行率研究。我们的内部ELISA最初使用79份血清样本进行验证，这79份血清样本在两种不同的商业ELISA试剂盒检测中结果一致。我们的ELISA表现出优异的灵敏度（94%）和特异性（100%），曲线下面积为0.99。进一步检测212份猪血清样本后发现，血清流行率为57.5%（95%置信区间，50.6 - 64.3%）。我们的研究结果表明，这种新型ELISA检测方法能够准确检测家猪体内的特异性抗HEV抗体，应在人类和其他哺乳动物中进一步验证。
