Klyosov A A, Gerasimas V B
Biochim Biophys Acta. 1979 Nov 9;571(1):162-5. doi: 10.1016/0005-2744(79)90237-7.
Thermostability of glucoamylase from Aspergillus niger is increased both by immobilization and substrate binding. However, the total superimposed stabilization effect at a given condition is apparently restricted by a certain limit which hardly depends on the mode of immobilization of the enzyme, and is determined mostly by the enzyme-substrate complex formation.
