Schmidt P, Rosenfeld E, Millner R, Schellenberger A
Institut für Angewandte Biophysik, Ber. Medizin, Martin-Luther-Universität Halle, Halle/Saale, GDR.
Ultrasonics. 1987 Sep;25(5):295-9. doi: 10.1016/0041-624x(87)90052-7.
The effect of ultrasonic waves on the activity of glucoamylase bound to a porous polystyrene matrix is investigated in this Paper. The immobilized enzyme was sonated in a flow cuvette at frequencies between 1 and 11 MHz and sound intensities up to 5 kW m-2. The effect was measured as a function of the type and concentration of the substrate, carrier particle size, flow rate of the substrate solution and ultrasonic frequency. The activity increase is discussed in terms of a possible ultrasonic mechanism.
本文研究了超声波对结合在多孔聚苯乙烯基质上的葡糖淀粉酶活性的影响。将固定化酶置于流动比色皿中,在1至11兆赫的频率和高达5千瓦每平方米的声强下进行超声处理。测定了该效应与底物类型和浓度、载体颗粒大小、底物溶液流速以及超声频率之间的函数关系。根据一种可能的超声作用机制对活性增加情况进行了讨论。