Changes of RNA mA/mC Modification Regulatory Molecules in Ferroptosis of T2DM Rat Pancreas.

N-methyladenine (mA) and 5-methylcytosine (mC) are two common forms of RNA methylation that play an important role in the epigenetics of type 2 diabetes mellitus (T2DM). One type of cell death, ferroptosis, has been implicated in islet β-cell damage in T2DM. Notably, RNA methylation, an upstream regulatory mechanism of mRNAs, can regulate the expression of ferroptosis signaling molecules, thereby affecting cell proliferation and death. Here, we found that the ferroptosis signaling pathway was activated in pancreas of T2DM rats, followed by significant changes in mA/mC modification regulatory molecules. These detection data together with the prediction results that mA and mC exist in the mRNAs of ferroptosis molecules, we speculate that mA and mC are probably involved in pancreatic cell damage by modifying of ferroptosis signaling molecules. In short, our findings provide a new research idea for future studies on the molecular mechanisms of pancreatic cell damage and point to a new direction for exploring the mechanisms of ferroptosis from the perspective of RNA methylation modification.