UCL Institute of Ophthalmology, London, UK.
National Institute for Health and Care Research Biomedical Research Centre at Moorfields Eye Hospital and UCL Institute of Ophthalmology, London, UK.
Methods Mol Biol. 2024;2800:67-74. doi: 10.1007/978-1-0716-3834-7_6.
The study of cell signaling within tissues can be enhanced using highly multiplexed immunohistochemistry to localize the presence and spatial distribution of numerous pathways of interest simultaneously. Additional data can also be gained by placing the identified proteins into the context of adjacent structures, stroma, and interacting partners. Here, we outline a protocol for using the recently described IBEX method on tissues. This is an open and simple cyclic immunohistochemistry approach suited to this application. We describe a simplified protocol and provide guidance on the method, using a 12-marker panel on human retina to demonstrate the approach.
在组织内研究细胞信号可以通过高度多重免疫组织化学来增强，从而同时定位许多感兴趣的途径的存在和空间分布。通过将鉴定的蛋白质置于相邻结构、基质和相互作用伙伴的背景中，还可以获得额外的数据。在这里，我们概述了一种在组织上使用最近描述的 IBEX 方法的方案。这是一种适用于此应用的开放且简单的循环免疫组织化学方法。我们描述了一个简化的方案，并使用人类视网膜的 12 标志物面板提供了方法指导，以展示该方法。
