Pescovitz M D, Lunney J K, Sachs D H
J Immunol. 1985 Jan;134(1):37-44.
Two murine monoclonal antibodies reactive with porcine nylon wool-nonadherent T cells were examined by flow microfluorometry analysis for cellular distribution, and by functional analysis for their effect on MLR and CTL assays. The two determinants recognized, called PT4 and PT8, were shown to be expressed on almost mutually exclusive cell populations, with only 6 to 15% of T cells expressing both antigens. Among the 15% of nylon wool-nonadherent cells that did not express either PT4 or PT8, 5% were surface Ig+. In functional assays, cells that proliferated to PHA, hen egg lysozyme, and alloantigens were blocked by PT4 and were eliminated by PT4 and complement. PT4 and complement had no effect on CTL effectors or precursors. Reciprocally, PT8 blocked and PT8 and complement eliminated CTL effectors and precursors, but had no effect on proliferative responses. These results indicate that PT4 and PT8 define the porcine helper and cytotoxic T cell subsets, respectively.
通过流式微量荧光分析检测了两种与猪尼龙毛非黏附性T细胞反应的鼠单克隆抗体的细胞分布，并通过功能分析检测了它们对混合淋巴细胞反应（MLR）和细胞毒性T淋巴细胞（CTL）检测的影响。所识别的两个决定簇，分别称为PT4和PT8，显示在几乎相互排斥的细胞群体上表达，只有6%至15%的T细胞同时表达这两种抗原。在不表达PT4或PT8的15%的尼龙毛非黏附细胞中，5%为表面免疫球蛋白阳性（surface Ig+）。在功能检测中，对PHA、鸡蛋清溶菌酶和同种异体抗原增殖的细胞被PT4阻断，并被PT4和补体清除。PT4和补体对CTL效应细胞或前体细胞没有影响。相反，PT8阻断且PT8和补体清除CTL效应细胞和前体细胞，但对增殖反应没有影响。这些结果表明，PT4和PT8分别定义了猪辅助性T细胞和细胞毒性T细胞亚群。
