Baranov V N, Rukosuev V S
Biull Eksp Biol Med. 1979 Oct;88(10):482-5.
The mouse liver was fixed by 12 fixator variants on the basis of glutaraldehyde, paraformaldehyde, picric acid and lysin, and the localization of albumin was studied by the indirect immunoperoxidase method. The comparison of the results shows that immersion in 8% paraformaldehyde is a preferable method of fixation. Albumin was detected on ribosomes and membranes of the nuclear envelope, the coarse endoplasmic reticulum and in the matrix of the hepatocytic cytoplasm. It is suggested that albumin synthetized is directly discharged into cytosol and then into the extracellular space.
采用基于戊二醛、多聚甲醛、苦味酸和赖氨酸的12种固定剂变体对小鼠肝脏进行固定，并通过间接免疫过氧化物酶法研究白蛋白的定位。结果比较表明，浸入8%多聚甲醛是一种较好的固定方法。在核糖体、核膜、粗面内质网和肝细胞细胞质基质中检测到白蛋白。推测合成的白蛋白直接排入细胞质溶胶，然后进入细胞外空间。
