LeBouton A V, Masse J P
Anat Rec. 1980 Jun;197(2):183-94. doi: 10.1002/ar.1091970207.
The cellular and subcellular localization of albumin in hepatocytes of adult male rats was established with immunofluorescence and immunoperoxidase techniques. Livers were fixed while either filled or devoid of blood. In some rats, prior treatment with cycloheximide was used to deplete the albumin content of hepatocytes. Immunofluorescence of blood-free livers from untreated rats showed that all hepatocytes contained albumin. However, using the peroxidase method, the amount of immunoprecipitate in cisternae of the rough endoplasmic reticulum was so slight that specific localization of albumin was impossible. Yet in all cases, a positive reaction for the presence of albumin was seen on ribosomes attached to the endoplasmic reticulum. In contrast, immunofluorescence of blood-filled livers from untreated rats and those previously injected with cycloheximide showed that only a few scattered hepatocytes were positive for albumin. In these cases, subcellular localization of albumin was obvious because the immunoprecipitate was found in heavy concentration, buy only in the cytosol compartment.
采用免疫荧光和免疫过氧化物酶技术确定成年雄性大鼠肝细胞中白蛋白的细胞和亚细胞定位。肝脏在充盈或无血状态下固定。在一些大鼠中，预先用环己酰亚胺处理以耗尽肝细胞中的白蛋白含量。未经处理大鼠的无血肝脏的免疫荧光显示所有肝细胞都含有白蛋白。然而，使用过氧化物酶方法，粗面内质网池中的免疫沉淀物量极少，以至于白蛋白的特异性定位无法实现。但在所有情况下，在内质网上附着的核糖体上都能看到白蛋白存在的阳性反应。相比之下，未经处理大鼠以及先前注射过环己酰亚胺的大鼠的充血肝脏的免疫荧光显示，只有少数散在的肝细胞对白蛋白呈阳性反应。在这些情况下，白蛋白的亚细胞定位很明显，因为免疫沉淀物大量集中存在，但仅在胞质溶胶区室中。
