Frontiers Science Center for Animal Breeding and Sustainable Production, Wuhan, China.
State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing, China.
Biotechnol Bioeng. 2024 Sep;121(9):2780-2792. doi: 10.1002/bit.28733. Epub 2024 May 6.
Pretreatment is crucial for effective enzymatic saccharification of lignocellulose such as sugarcane bagasse (SCB). In the present study, SCB was pretreated with five kinds of heterogeneous Fenton-like systems (HFSs), respectively, in which α-FeOOH, α-FeO, FeO, and FeS worked as four traditional heterogeneous Fenton-like catalysts (HFCs), while FeVO worked as a novel HFC. The enzymatic reducing sugar conversion rate was then compared among SCB after different heterogeneous Fenton-like pretreatments (HFPs), and the optimal HFS and pretreatment conditions were determined. The mechanism underlying the difference in saccharification efficiency was elucidated by analyzing the composition and morphology of SCB. Moreover, the ion dissolution characteristics, variation of pH and Eh values, HO and hydroxyl radical (·OH) concentration of FeVO and α-FeO HFSs were compared. The results revealed that the sugar conversion rate of SCB pretreated with FeVO HFS reached up to 58.25%, which was obviously higher than that under other HFPs. In addition, the surface morphology and composition of the pretreated SCB with FeVO HFS were more conducive to enzymatic saccharification. Compared with α-FeO, FeVO could utilize HO more efficiently, since the dissolved Fe and V can both react with HO to produce more ·OH, resulting in a higher hemicellulose and lignin removal rate and a higher enzymatic sugar conversion rate. It can be concluded that FeVO HFP is a promising approach for lignocellulose pretreatment.
预处理对于甘蔗渣（SCB）等木质纤维素的有效酶糖化至关重要。在本研究中，分别使用五种非均相芬顿样系统（HFS）对 SCB 进行预处理，其中α-FeOOH、α-FeO、FeO 和 FeS 作为四种传统的非均相芬顿样催化剂（HFC），而 FeVO 作为一种新型 HFC。然后比较了 SCB 在不同非均相芬顿样预处理（HFPs）后的酶还原糖转化率，并确定了最佳 HFS 和预处理条件。通过分析 SCB 的组成和形态，阐明了糖化效率差异的机制。此外，还比较了 FeVO 和α-FeO HFS 的离子溶解特性、pH 值和 Eh 值的变化、HO 和羟基自由基（·OH）浓度。结果表明，用 FeVO HFS 预处理的 SCB 的糖转化率高达 58.25%，明显高于其他 HFPs。此外，用 FeVO HFS 预处理的 SCB 的表面形态和组成更有利于酶糖化。与 FeO 相比，FeVO 可以更有效地利用 HO，因为溶解的 Fe 和 V 都可以与 HO 反应生成更多的·OH，从而导致更高的半纤维素和木质素去除率和更高的酶糖转化率。可以得出结论，FeVO HFP 是一种很有前途的木质纤维素预处理方法。
