Hoessli D, Rungger-Brändle E
Exp Cell Res. 1985 Jan;156(1):239-50. doi: 10.1016/0014-4827(85)90278-2.
A non-ionic detergent-resistant complex of membrane-associated proteins and cell-surface glycoproteins has been isolated by gel filtration and isopyknic centrifugation of purified plasma membranes from the murine T-lymphoma P 1798. This complex elutes as a high molecular weight peak (greater than 15 X 10(6) D) and contains two specific sets of (1) cell-surface glycoproteins; (2) membrane-associated proteins. The cell-surface glycoproteins consist of two vectorially labelled major components present in a fixed molar ratio: The Thy-1 glycoprotein and a non-H-2 glycoprotein of 55 kD. Minor but significant amounts of the class I histocompatibility antigen Qa-2 are also contained in the detergent-resistant complex. The membrane-associated proteins are not vectorially labelled, and form a complex group of proteins in the 30-70 kD range. Since actin is not detectable among these polypeptides, they probably constitute a plasma membrane-associated structure that is distinct from actin-containing, submembranous cytoskeletal elements.
通过凝胶过滤和等密度离心法，从鼠T淋巴瘤P 1798中分离出纯化的质膜，得到了一种与膜相关蛋白和细胞表面糖蛋白形成的抗非离子去污剂复合物。该复合物以高分子量峰（大于15×10⁶ D）洗脱，包含两组特定的物质：（1）细胞表面糖蛋白；（2）膜相关蛋白。细胞表面糖蛋白由两种以固定摩尔比存在的主要成分组成：Thy-1糖蛋白和一种55 kD的非H-2糖蛋白。抗去污剂复合物中还含有少量但显著量的I类组织相容性抗原Qa-2。膜相关蛋白没有进行向量标记，形成了一组分子量在30 - 70 kD范围内的复杂蛋白质。由于在这些多肽中未检测到肌动蛋白，它们可能构成了一种与含肌动蛋白的膜下细胞骨架元件不同的质膜相关结构。
