Pinho R T, de Simone S G
Departamento de Imunologia, Instituto Oswaldo Cruz, Rio de Janeiro, Brasil.
Mem Inst Oswaldo Cruz. 1989 Jan-Mar;84(1):13-8. doi: 10.1590/s0074-02761989000100004.
Cell surface proteins of Trypanosoma dionisii, Trypanosoma vespertilionis and Trypanosoma sp. (M238) were radiodinated and their distribution both in the detergent-poor (DPP) and detergent-enriched phase (DRP) was studied using a phase separation technique in Triton X-114, as well as polyacrylamide gel electrophoresis in sodium dodecyl sulphate (SDS-PAGE). Significant differences were observed in the proteins present in the DRP when the three species of trypanosoma were compared. Two major bands with 88 and 70 KDa were observed in T. sp. (M238) but were not detectable in T. dionisii and T. vespertilionis. Three polypeptides with 96, 77 and 60 KDa were identified in the DRP of T. vespertilionis. Three major bands with 84, 72 and 60 KDa were observed in the DRP of T. dionisii. Two polypeptides with 34-36 KDa present in the DPP, were observed in the three Trypanosome species analyzed. Our observations show that T. sp. (M238) has characteristic surface polypeptides not found in T. dionisii and T. vespertilionis.
对狄氏锥虫、蝙蝠锥虫和锥虫属（M238）的细胞表面蛋白进行放射性碘化，并使用Triton X - 114相分离技术以及十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS - PAGE）研究其在贫去污剂相（DPP）和富去污剂相（DRP）中的分布。比较这三种锥虫时，在DRP中存在的蛋白质上观察到显著差异。在锥虫属（M238）中观察到两条主要条带，分子量分别为88 kDa和70 kDa，但在狄氏锥虫和蝙蝠锥虫中未检测到。在蝙蝠锥虫的DRP中鉴定出三条分子量分别为96 kDa、77 kDa和60 kDa的多肽。在狄氏锥虫的DRP中观察到三条主要条带，分子量分别为84 kDa、72 kDa和60 kDa。在分析的三种锥虫的DPP中均观察到两条分子量为34 - 36 kDa的多肽。我们的观察结果表明，锥虫属（M238）具有狄氏锥虫和蝙蝠锥虫中未发现的特征性表面多肽。
