Centre Hospitalier Universitaire Régional de Ouahigouya, Ouahigouya, Burkina Faso.
Université Nazi Boni, Bobo-Dioulasso, Burkina Faso.
Mycoses. 2024 May;67(5):e13732. doi: 10.1111/myc.13732.
Triazole-resistant Aspergillus fumigatus (TRAF) isolates are a growing public health problem with worldwide distribution. Epidemiological data on TRAF is limited in Africa, particularly in West Africa.
This study aimed to screen for the environmental presence of TRAF isolates in the indoor air of two hospitals in Burkina Faso.
Air samples were collected in wards housing patients at risk for invasive aspergillosis, namely infectious diseases ward, internal medicine ward, nephrology ward, pulmonology ward, medical emergency ward and paediatric ward. Sabouraud Dextrose Agar supplemented with triazoles was used to screen the suspected TRAF isolates and EUCAST method to confirm the resistance of suspected isolates. Sequencing of cyp51A gene was used to identify the resistance mechanism of confirmed TRAF isolates.
Of the 198 samples collected and analysed, 67 showed growth of A. fumigatus isolates. The prevalence of TRAF isolates was 3.23% (4/124). One TRAF isolate exhibited a pan-triazole resistance. Sequencing of cyp51A gene identified the TR34/L98H mutation for this pan-triazole resistant isolate. This study showed for the first time the circulation of the pan-azole resistant isolate harbouring the TR34/L98H mutation in Burkina Faso.
These findings emphasise the need to map these TRAF isolates in all parts of Burkina Faso and to establish local and national continuous surveillance of environmental and clinical TRAF isolates in this country.
具有三唑类耐药性的烟曲霉（TRAF）分离株是一个日益严重的全球公共卫生问题。在非洲，尤其是在西非，TRAF 的流行病学数据有限。
本研究旨在筛查布基纳法索两家医院室内空气中 TRAF 分离株的环境存在情况。
在易发生侵袭性曲霉病的病房（传染病病房、内科病房、肾病病房、肺病病房、急诊医学病房和儿科病房）采集患者所在病房的空气样本。用添加三唑类药物的沙氏葡萄糖琼脂筛选疑似 TRAF 分离株，用 EUCAST 方法确认疑似分离株的耐药性。对确证的 TRAF 分离株进行 cyp51A 基因测序，以确定耐药机制。
在采集和分析的 198 个样本中，有 67 个显示出烟曲霉分离株的生长。TRAF 分离株的流行率为 3.23%（4/124）。1 株 TRAF 分离株表现出泛三唑耐药性。cyp51A 基因测序确定了该泛三唑耐药分离株的 TR34/L98H 突变。本研究首次在布基纳法索发现携带 TR34/L98H 突变的泛唑耐药分离株。
这些发现强调了在布基纳法索各地绘制这些 TRAF 分离株图谱的必要性，并在该国建立环境和临床 TRAF 分离株的地方和国家持续监测。
