Department of Biochemistry, Institute of Science.
Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh.
J Infect Dis. 2024 Jun 14;229(6):1909-1912. doi: 10.1093/infdis/jiae156.
In an area endemic with Indian visceral leishmaniasis (VL), we performed direct xenodiagnosis to evaluate the transmission of Leishmania donovani from patients with VL-human immunodeficiency virus (HIV) coinfection to the vector sandflies, Phlebotomus argentipes. Fourteen patients with confirmed VL-HIV coinfection, with a median parasitemia of 42 205 parasite genome/mL of blood, were exposed to 732 laboratory-reared pathogen-free female P argentipes sandflies on their lower arms and legs. Microscopy revealed that 16.66% (122/732) of blood-fed flies were xenodiagnosis positive. Notably, 93% (13/14) of the VL-HIV group infected the flies, as confirmed by quantitative polymerase chain reaction and/or microscopy, and were 3 times more infectious than those who had VL without HIV.
在印度内脏利什曼病（VL）流行地区，我们进行了直接异种诊断，以评估从患有 VL-人类免疫缺陷病毒（HIV）合并感染的患者向媒介白蛉传播利什曼原虫 Donovan 的情况。我们让 14 名经证实患有 VL-HIV 合并感染的患者，他们的中位寄生虫血症为 42205 个寄生虫基因组/mL 的血液，将其下臂和腿部暴露于 732 只实验室饲养的无病原体雌性白蛉属（Phlebotomus argentipes）沙蝇。显微镜检查显示，16.66%（122/732）的吸血苍蝇呈异种诊断阳性。值得注意的是，93%（13/14）的 VL-HIV 组感染了苍蝇，这通过定量聚合酶链反应和/或显微镜检查得到了证实，其感染性比没有 HIV 的 VL 患者高 3 倍。
