Morse J H, Carlino J A, Lazzarino D, Meng T C
Clin Immunol Immunopathol. 1985 Feb;34(2):237-53. doi: 10.1016/0090-1229(85)90028-5.
Experiments were designed to examine the effects of pregnancy-associated growth factor (PAGF), a substance found in commercial preparations of crude human chorionic gonadotropin (hCG), on unfractionated human cord blood cells (CBC) and adult peripheral blood lymphocytes (PBL) cultured in 5% fetal calf serum (FCS). Comparisons of PAGF-induced [3H]TdR incorporation in nine pairs of simultaneously cultured CBC and PBL with phytohemagglutinin (PHA) and tetanus toxoid (TT) showed that all CBC and PBL responded to PAGF and PHA whereas all PBL and one CBC responded to TT. The rank order of potency for CBC and PBL was PHA greater than PAGF greater than TT. To examine phenotypic changes induced by PAGF, flow cytometry was performed on precultured cells, control cultures, and PAGF-stimulated cultures at 2, 5, 7, and 9 days. The monoclonal antibodies (mAbs) included T3, T4, and T8 (T cells), T9 (transferrin receptor), Tac (IL-2 receptor), 12 (Ia or DR-framework antigen), and T10 (putative activation and/or maturation antigen). PAGF-stimulated cultures had statistically significant increased percentages of T3, T4, T9, T10, and Tac but not T8 when compared to precultured cells and control cultures. PAGF also increased PBL but not CBC Ia. In PAGF-stimulated cultures, CBC had more T3 and T4 cells with increased fluorescence intensity than PBL. Maximal expression of phenotypes usually occurred at Days 7 and 9, 2 days after maximal [3H]TdR incorporation. In comparison to PAGF, PHA-stimulated PBL had earlier expression of these phenotypes but included T8. These data indicate PAGF induces proliferation, activation antigens, and T3 expansion predominantly confined to the T4 subset in both CBC and PBL.
实验旨在研究妊娠相关生长因子（PAGF）对在5%胎牛血清（FCS）中培养的未分级人脐带血细胞（CBC）和成人外周血淋巴细胞（PBL）的影响，PAGF是在粗制人绒毛膜促性腺激素（hCG）商业制剂中发现的一种物质。对九对同时培养的CBC和PBL中PAGF诱导的[3H]TdR掺入量与植物血凝素（PHA）和破伤风类毒素（TT）进行比较，结果显示所有CBC和PBL对PAGF和PHA有反应，而所有PBL和一对CBC对TT有反应。CBC和PBL的效力排序为PHA大于PAGF大于TT。为了研究PAGF诱导的表型变化，在第2、5、7和9天对预培养细胞、对照培养物和PAGF刺激的培养物进行了流式细胞术检测。单克隆抗体（mAb）包括T3、T4和T8（T细胞）、T9（转铁蛋白受体）、Tac（IL-2受体）、12（Ia或DR框架抗原）和T10（假定的活化和/或成熟抗原）。与预培养细胞和对照培养物相比，PAGF刺激的培养物中T3、T4、T9、T10和Tac的百分比有统计学意义的增加，但T8没有。PAGF还增加了PBL的Ia，但没有增加CBC的Ia。在PAGF刺激的培养物中，CBC的T3和T4细胞比PBL更多，且荧光强度增加。表型的最大表达通常发生在第7天和第9天，即[3H]TdR掺入量最大后的2天。与PAGF相比，PHA刺激的PBL这些表型的表达更早，但包括T8。这些数据表明PAGF诱导增殖、活化抗原以及T3扩增，主要局限于CBC和PBL中的T4亚群。
