Laboratorio Nacional para la Investigación en Inocuidad Alimentaria (LANIIA), Centro de Investigación en Alimentación y Desarrollo, A.C. (CIAD), Carretera a Eldorado km 5.5, Campo El Diez, 80110, Culiacan, Sinaloa, Mexico.
Facultad de Ciencias de la Nutrición y Gastronomía, Universidad Autónoma de Sinaloa, 80019, Culiacan, Sinaloa, Mexico.
Virology. 2024 Jul;595:110100. doi: 10.1016/j.virol.2024.110100. Epub 2024 May 3.
Enterobacter cloacae is a clinically significant pathogen due to its multi-resistance to antibiotics, presenting a challenge in the treatment of infections. As concerns over antibiotic resistance escalate, novel therapeutic approaches have been explored. Bacteriophages, characterized by their remarkable specificity and ability to self-replicate within target bacteria, are emerging as a promising alternative therapy. In this study, we isolated and partially characterized nine lytic bacteriophages targeting E. cloacae, with two selected for comprehensive genomic analysis based on their host range and bacteriolytic activity. All identified phages exhibited a narrow host range, demonstrated stability within a temperature range of 30-60 °C, displayed pH tolerance from 3 to 10, and showed an excellent bacteriolytic capacity for up to 18 h. Notably, the fully characterized phage genomes revealed an absence of lysogenic, virulence, or antibiotic-resistance genes, positioning them as promising candidates for therapeutic intervention against E. cloacae-related diseases. Nonetheless, translating this knowledge into practical therapeutic applications mandates a deeper understanding of bacteriophage interactions within complex biological environments.
阴沟肠杆菌是一种具有临床意义的病原体，由于其对多种抗生素的耐药性，给感染的治疗带来了挑战。随着对抗生素耐药性的担忧加剧，人们探索了新的治疗方法。噬菌体具有显著的特异性和在目标细菌内自我复制的能力，作为一种有前途的替代治疗方法正在出现。在这项研究中，我们分离并部分鉴定了针对阴沟肠杆菌的 9 种溶菌噬菌体，其中两种根据其宿主范围和溶菌活性被选择进行全面基因组分析。所有鉴定出的噬菌体都表现出狭窄的宿主范围，在 30-60°C 的温度范围内稳定，在 pH 值为 3 到 10 的范围内耐受，并且在长达 18 小时内具有出色的溶菌能力。值得注意的是，完全鉴定的噬菌体基因组显示出不存在溶原性、毒性或抗生素耐药性基因，这使它们成为治疗阴沟肠杆菌相关疾病的有前途的候选者。然而，将这些知识转化为实际的治疗应用需要更深入地了解噬菌体在复杂生物环境中的相互作用。
