Institute of Natural Antioxidants and Anti-Inflammation, Dali University, Dali, 671003, Yunnan, China.
Institute of Eastern-Himalaya Biodiversity Research, Dali University, Dali, 671003, Yunnan, China.
Sci Rep. 2024 May 7;14(1):10509. doi: 10.1038/s41598-024-61012-7.
Chronic non-communicable diseases (CNCDs) pose a significant public health challenge. Addressing this issue, there has been a notable breakthrough in the prevention and mitigation of NCDs through the use of antioxidants and anti-inflammatory agents. In this study, we aim to explore the effectiveness of Eupatorium adenophora Spreng leaves (EASL) as an antioxidant and anti-inflammatory agent, and its potential applications. To construct a cellular model of oxidative damage and inflammation, Caco-2 cells were treated with tert-butyl hydroperoxide (t-BHP). The biocompatibility of EASL-AE with Caco-2 cells was assessed using the MTT assay, while compatibility was further verified by measuring LDH release and the protective effect against oxidative damage was also assessed using the MTT assay. Additionally, we measured intracellular oxidative stress indicators such as ROS and 8-OHdG, as well as inflammatory pathway signalling protein NFκB and inflammatory factors TNF-α and IL-1β using ELISA, to evaluate the antioxidant and anti-inflammatory capacity of EASL-AE. The scavenging capacity of EASL-AE against free radicals was determined through the DPPH Assay and ABTS Assay. Furthermore, we measured the total phenolic, total flavonoid, and total polysaccharide contents using common chemical methods. The chemical composition of EASL-AE was analyzed using the LC-MS/MS technique. Our findings demonstrate that EASL-AE is biocompatible with Caco-2 cells and non-toxic at experimental levels. Moreover, EASL-AE exhibits a significant protective effect on Caco-2 cells subjected to oxidative damage. The antioxidant effect of EASL-AE involves the scavenging of intracellular ROS, while its anti-inflammatory effect is achieved by down-regulation of the NFκB pathway. Which in turn reduces the release of inflammatory factors TNF-α and IL-1β. Through LC-MS/MS analysis, we identified 222 compounds in EASL-AE, among which gentianic acid, procaine and L-tyrosine were the compounds with high antioxidant capacity and may be the effective constituent for EASL-AE with antioxidant activity. These results suggest that EASL-AE is a natural and high-quality antioxidant and anti-inflammatory biomaterial that warrants further investigation. It holds great potential for applications in healthcare and other related fields.
慢性非传染性疾病（CNCDs）是一个重大的公共卫生挑战。在预防和减轻非传染性疾病方面，抗氧化剂和抗炎剂的使用取得了显著突破。在这项研究中，我们旨在探讨紫茎泽兰叶片（EASL）作为抗氧化剂和抗炎剂的有效性及其潜在应用。为构建氧化损伤和炎症的细胞模型，用叔丁基过氧化氢（t-BHP）处理 Caco-2 细胞。采用 MTT 法评估 EASL-AE 与 Caco-2 细胞的生物相容性，进一步通过测量 LDH 释放验证其相容性，并通过 MTT 法评估其对氧化损伤的保护作用。此外，我们还通过 ELISA 测量细胞内氧化应激指标，如 ROS 和 8-OHdG，以及炎症途径信号蛋白 NFκB 和炎症因子 TNF-α和 IL-1β，以评估 EASL-AE 的抗氧化和抗炎能力。通过 DPPH 法和 ABTS 法测定 EASL-AE 对自由基的清除能力。此外，我们还采用常见的化学方法测定 EASL-AE 的总酚、总黄酮和总多糖含量。采用 LC-MS/MS 技术分析 EASL-AE 的化学成分。我们的研究结果表明，EASL-AE 与 Caco-2 细胞具有生物相容性，在实验水平下无毒性。此外，EASL-AE 对遭受氧化损伤的 Caco-2 细胞具有显著的保护作用。EASL-AE 的抗氧化作用涉及清除细胞内 ROS，而其抗炎作用是通过下调 NFκB 途径实现的。这反过来又减少了炎症因子 TNF-α和 IL-1β的释放。通过 LC-MS/MS 分析，我们在 EASL-AE 中鉴定出 222 种化合物，其中龙胆酸、普鲁卡因和 L-酪氨酸是具有高抗氧化能力的化合物，可能是具有抗氧化活性的 EASL-AE 的有效成分。这些结果表明，EASL-AE 是一种天然的、高质量的抗氧化和抗炎生物材料，值得进一步研究。它在医疗保健和其他相关领域具有广阔的应用前景。
