Li Zheng-Qiang, Li Rui, Deng Wei, An Rui, Ma Ping, Li Jun, She Rong, Yang Xiao-Yan, Yang Xu, Xiao Wen
Institute of Natural Antioxidants and Anti-Inflammation, Dali University, Dali, 671003, China.
Institute of Eastern-Himalaya Biodiversity Research, Dali University, Dali, 671003, China.
Probiotics Antimicrob Proteins. 2025 Jul 18. doi: 10.1007/s12602-025-10655-7.
The health benefits of probiotics are widely acknowledged, and the development of probiotics with antioxidant and anti-inflammatory properties is expected to further advance the probiotic industry and enhance human health. This study reports a novel lactic acid strain, Clostridium dalinum, isolated from soil. As a member of the Clostridium genus, this strain demonstrates strong coagulation properties and a high safety profile. Notably, C. dalinum exhibits exceptional gastrointestinal tolerance, retaining viability under acidic conditions (pH 3.0 for 2 h) and high bile stress (3% bile for 3 h). Evaluation using Caco-2 cells confirmed that both the strain and the yogurt fermented with it exhibit antioxidant and anti-inflammatory functions mediated through the NF-κB and Keap1-Nrf2-ARE signaling pathways. Metabolomics analysis identified significantly elevated levels of antioxidant and anti-inflammatory metabolites including Chavicol, Neocnidilide, Procaine, and 6-Phosphogluconic acid in the C. dalinum group, functionally linked to amino acid, lipid, and cofactor metabolism pathways. Pathway enrichment analysis further demonstrated specific activation of butanoate metabolism by C. dalinum. In the D-yogurt group, key metabolites were similarly upregulated, while purine metabolism was uniquely downregulated. These findings confirm that C. dalinum is a safe probiotic with notable antioxidant and anti-inflammatory properties, demonstrating potential for immune modulation and therapeutic applications in intestinal damage and inflammation. Importantly, this discovery offers a novel perspective for probiotic development.
益生菌对健康的益处已得到广泛认可，开发具有抗氧化和抗炎特性的益生菌有望进一步推动益生菌产业发展并增进人类健康。本研究报告了一种从土壤中分离出的新型乳酸菌株——达林梭菌。作为梭菌属的一员，该菌株具有很强的凝结特性且安全性高。值得注意的是，达林梭菌具有出色的胃肠道耐受性，在酸性条件下（pH 3.0，2小时）和高胆汁压力下（3%胆汁，3小时）仍能保持活力。使用Caco-2细胞进行的评估证实，该菌株及其发酵的酸奶均通过NF-κB和Keap1-Nrf2-ARE信号通路发挥抗氧化和抗炎功能。代谢组学分析确定，达林梭菌组中抗氧化和抗炎代谢物（包括丁香酚、新蛇床内酯、普鲁卡因和6-磷酸葡萄糖酸）的水平显著升高，这些代谢物在功能上与氨基酸、脂质和辅因子代谢途径相关。通路富集分析进一步表明达林梭菌对丁酸代谢有特异性激活作用。在D-酸奶组中，关键代谢物同样上调，而嘌呤代谢则独特地下调。这些发现证实，达林梭菌是一种安全的益生菌，具有显著的抗氧化和抗炎特性，在肠道损伤和炎症的免疫调节及治疗应用方面具有潜力。重要的是，这一发现为益生菌开发提供了新的视角。
