State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Guangzhou, 510060, China.
MOE Key Laboratory of Gene Function and Regulation, Guangdong Province Key Laboratory of Pharmaceutical Functional Genes, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China.
BMC Biol. 2024 May 7;22(1):106. doi: 10.1186/s12915-024-01908-y.
The significance of A-to-I RNA editing in nervous system development is widely recognized; however, its influence on retina development remains to be thoroughly understood.
In this study, we performed RNA sequencing and ribosome profiling experiments on developing mouse retinas to characterize the temporal landscape of A-to-I editing. Our findings revealed temporal changes in A-to-I editing, with distinct editing patterns observed across different developmental stages. Further analysis showed the interplay between A-to-I editing and alternative splicing, with A-to-I editing influencing splicing efficiency and the quantity of splicing events. A-to-I editing held the potential to enhance translation diversity, but this came at the expense of reduced translational efficiency. When coupled with splicing, it could produce a coordinated effect on gene translation.
Overall, this study presents a temporally resolved atlas of A-to-I editing, connecting its changes with the impact on alternative splicing and gene translation in retina development.
A-to-I RNA 编辑在神经系统发育中的意义已得到广泛认可；然而，其对视网膜发育的影响仍有待深入理解。
本研究通过对发育中的小鼠视网膜进行 RNA 测序和核糖体分析实验，对 A-to-I 编辑的时空特征进行了研究。我们的研究结果显示，A-to-I 编辑存在时间变化，不同发育阶段观察到不同的编辑模式。进一步的分析表明，A-to-I 编辑与可变剪接之间存在相互作用，A-to-I 编辑影响剪接效率和剪接事件的数量。A-to-I 编辑有可能增强翻译多样性，但这是以降低翻译效率为代价的。当与剪接结合时，它可以对基因翻译产生协调效应。
总的来说，本研究提供了一个具有时间分辨率的 A-to-I 编辑图谱，将其变化与视网膜发育中对可变剪接和基因翻译的影响联系起来。
