评估两种正布尼亚病毒（Oya 病毒和伊宁市湖病毒）的蚊媒传播范围。

Evaluating the mosquito vector range for two orthobunyaviruses: Oya virus and Ebinur Lake virus.

机构信息

Key Laboratory of Virology and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, Hubei, China.

University of Chinese Academy of Sciences, Beijing, China.

出版信息

Parasit Vectors. 2024 May 7;17(1):204. doi: 10.1186/s13071-024-06295-5.

DOI:10.1186/s13071-024-06295-5

PMID:38715075

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11077878/

Abstract

BACKGROUND

Mosquito-borne viruses cause various infectious diseases in humans and animals. Oya virus (OYAV) and Ebinur Lake virus (EBIV), belonging to the genus Orthobunyavirus within the family Peribunyaviridae, are recognized as neglected viruses with the potential to pose threats to animal or public health. The evaluation of vector competence is essential for predicting the arbovirus transmission risk.

METHODS

To investigate the range of mosquito vectors for OYAV (strain SZC50) and EBIV (strain Cu20-XJ), the susceptibility of four mosquito species (Culex pipiens pallens, Cx. quinquefasciatus, Aedes albopictus, and Ae. aegypti) was measured through artificial oral infection. Then, mosquito species with a high infection rate (IR) were chosen to further evaluate the dissemination rate (DR), transmission rate (TR), and transmission efficiency. The viral RNA in each mosquito sample was determined by RT-qPCR.

RESULTS

The results revealed that for OYAV, Cx. pipiens pallens had the highest IR (up to 40.0%) among the four species, but the DR and TR were 4.8% and 0.0%, respectively. For EBIV, Cx. pipiens pallens and Cx. quinquefasciatus had higher IR compared to Ae. albopictus (1.7%). However, the EBIV RNA and infectious virus were detected in Cx. pipiens pallens, with a TR of up to 15.4% and a transmission efficiency of 3.3%.

CONCLUSIONS

The findings indicate that Cx. pipiens pallens was susceptible to OYAV but had an extremely low risk of transmitting the virus. Culex pipiens pallens and Cx. quinquefasciatus were susceptible to EBIV, and Cx. pipiens pallens had a higher transmission risk to EBIV than Cx. quinquefasciatus.

摘要

背景

蚊媒病毒可引起人类和动物的各种传染病。Oya 病毒（OYAV）和额尔齐斯河病毒（EBIV）属于布尼亚病毒科白蛉病毒属，是被忽视的病毒，它们有可能对动物或公共卫生构成威胁。评估媒介的感染能力对于预测虫媒病毒的传播风险至关重要。

方法

为了研究 OYAV（SZC50 株）和 EBIV（Cu20-XJ 株）的蚊媒范围，通过人工经口感染测定了 4 种蚊种（白纹伊蚊、致倦库蚊、埃及伊蚊和三带喙库蚊）的易感性。然后，选择感染率高（IR）的蚊种进一步评估其扩散率（DR）、传播率（TR）和传播效率。用 RT-qPCR 检测每个蚊样中的病毒 RNA。

结果

结果表明，对于 OYAV，4 种蚊种中白纹伊蚊的 IR 最高（高达 40.0%），但 DR 和 TR 分别为 4.8%和 0.0%。对于 EBIV，白纹伊蚊和致倦库蚊的 IR 高于埃及伊蚊（1.7%）。然而，EBIV RNA 和感染性病毒在白纹伊蚊中被检测到，TR 高达 15.4%，传播效率为 3.3%。