Laurent P, Bieth J G
Biochem Biophys Res Commun. 1985 Jan 16;126(1):275-81. doi: 10.1016/0006-291x(85)90602-3.
This paper describes a non-oxidative impairment of the biological function of alpha 1-proteinase inhibitor by cigarette smoke. Aqueous solutions of cigarette smoke are able to decrease the rate constant kass for the inhibition of porcine pancreatic elastase by human plasma alpha 1-proteinase inhibitor. The value of kass decreases linearly with the concentration of smoke (from 2.2 X 10(5) M-1 s-1 to 0.6 X 10(5) M-1 s-1). This effect is not due to an oxidation of the inhibitor. When pancreatic elastase is reacted with elastin in the presence of alpha 1-proteinase inhibitor and cigarette smoke solution, elastolysis occurs at a rate nearly identical to that observed in the absence of inhibitor. This effect is due to a smoke-induced decrease in kass. These observations may serve as a model of biological regulation of proteolysis via a change in the rate constant for a proteinase-proteinase inhibitor association. The influence of cigarette smoke on the inhibition of human neutrophil elastase by alpha 1-proteinase inhibitor could not be studied in detail because the enzyme precipitates in the presence of concentrated smoke solution.
本文描述了香烟烟雾对α1-蛋白酶抑制剂生物学功能的非氧化损伤。香烟烟雾的水溶液能够降低人血浆α1-蛋白酶抑制剂抑制猪胰弹性蛋白酶的速率常数kass。kass值随烟雾浓度呈线性下降（从2.2×10⁵M⁻¹s⁻¹降至0.6×10⁵M⁻¹s⁻¹）。这种效应并非由于抑制剂的氧化。当在α1-蛋白酶抑制剂和香烟烟雾溶液存在的情况下，胰弹性蛋白酶与弹性蛋白反应时，弹性蛋白分解的速率与在无抑制剂时观察到的速率几乎相同。这种效应是由于烟雾导致kass降低。这些观察结果可作为通过蛋白酶-蛋白酶抑制剂结合速率常数变化来进行蛋白水解生物学调节的模型。由于该酶在浓烟雾溶液存在下会沉淀，因此无法详细研究香烟烟雾对α1-蛋白酶抑制剂抑制人中性粒细胞弹性蛋白酶的影响。
