Janoff A, Dearing R
Am Rev Respir Dis. 1982 Oct;126(4):691-4. doi: 10.1164/arrd.1982.126.4.691.
Aqueous solutions of gas phase cigarette smoke were incubated with pure human leukocyte elastase or with crude human leukocyte granule extract, and the effects on enzyme activity were determined using a synthetic amide substrate. Simultaneously, the same smoke solutions were incubated with 10% human serum under identical conditions, and the effects on serum inhibition of purified or crude leukocyte elastase were similarly measured. In addition, aqueous solutions of unfractionated cigarette smoke were incubated with leukocyte elastase or serum, and the abilities of the smoke-treated enzyme to digest elastin and of the smoke-treated serum to inhibit elastin digestion were determined. Both experimental protocols showed that serum elastase-inhibiting capacity (primarily caused by alpha 1-proteinase inhibitor) is more susceptible to inactivation by aqueous solutions of cigarette smoke than is leukocyte elastase, suggesting that elastase inhibition (rather than elastase activity) may be predominantly suppressed by cigarette smoke inhalation in vivo.
将气相香烟烟雾的水溶液与纯人白细胞弹性蛋白酶或粗制人白细胞颗粒提取物一起孵育，使用合成酰胺底物测定对酶活性的影响。同时，在相同条件下将相同的烟雾溶液与10%人血清一起孵育，同样测量对纯化或粗制白细胞弹性蛋白酶的血清抑制作用的影响。此外，将未分级香烟烟雾的水溶液与白细胞弹性蛋白酶或血清一起孵育，测定经烟雾处理的酶消化弹性蛋白的能力以及经烟雾处理的血清抑制弹性蛋白消化的能力。两种实验方案均表明，血清弹性蛋白酶抑制能力（主要由α1-蛋白酶抑制剂引起）比白细胞弹性蛋白酶更容易被香烟烟雾的水溶液灭活，这表明在体内吸入香烟烟雾可能主要抑制弹性蛋白酶抑制作用（而非弹性蛋白酶活性）。
