Endo Y, Suzuki R, Kumagai K
Biochim Biophys Acta. 1985 Mar 8;838(3):343-50. doi: 10.1016/0304-4165(85)90232-6.
Injection of culture medium of P388D1 cells, a murine macrophage cell line, into mice produced a rapid induction of ornithine decarboxylase in the liver and spleen. The ornithine decarboxylase-inducing factor in the medium was purified by gel-filtration and chromatofocusing. With these procedures, the factor was usually found in the fraction of interleukin 1 (a lymphocyte-activating factor), indicating that the factor is identical with interleukin 1 or a molecule closely related to interleukin 1. The ornithine decarboxylase induction by the factor was suppressed by cycloheximide, but actinomycin D did not suppress the induction, or rather enhanced it. These findings may provide valuable information on the important roles of macrophages in immune responses or in inflammatory reactions.
将鼠巨噬细胞系P388D1细胞的培养基注射到小鼠体内，可迅速诱导肝脏和脾脏中的鸟氨酸脱羧酶。通过凝胶过滤和色谱聚焦法对培养基中的鸟氨酸脱羧酶诱导因子进行了纯化。通过这些步骤，该因子通常存在于白细胞介素1（一种淋巴细胞激活因子）的组分中，这表明该因子与白细胞介素1相同或与白细胞介素1密切相关的分子。该因子诱导的鸟氨酸脱羧酶被放线菌酮抑制，但放线菌素D不抑制诱导，反而增强诱导。这些发现可能为巨噬细胞在免疫反应或炎症反应中的重要作用提供有价值的信息。
