Furukawa F, Maruyama N, Yoshida H, Hamashima Y, Hirose S, Shirai T
Clin Exp Immunol. 1985 Jan;59(1):146-52.
We found that the lupus band test (LBT) is frequently positive in the tail skin in NZB and NZB X NZW (B/W) F1 hybrid mice. Genetic mode of development of this positivity was investigated in female NZB, NZW, B/W F1 hybrid and B/W F1 X NZW back-cross mice and the incidences were 60%, 0%, 100% and 42% by 12 months of age, respectively. It was found that B/W F1 hybrid mice showed not only a higher incidence but also an earlier onset of LBT positivity than did the parental NZB mice. These findings suggested that a single dominant locus in NZB strain determines the appearance of a positive LBT and that this trait is to a great degree intensified by the involvement of NZW gene(s) in B/W F1 hybrid mice. Linkage studies indicated that the NZB gene is to some extent linked to H-2 complex. In addition, there were significant associations between the LBT positivity and the appearances of anti-dsDNA antibodies and renal disease in B/W F1 X NZW back-cross mice. Thus, it is suggested that the NZB-LBT gene is the gene itself or closely linked, on chromosome 17, to that related in part to the occurrence of anti-DNA antibodies and renal disease. There was a lack of retroviral gp70 deposition at the dermo-epidermal junction in NZB and B/W F1 hybrid mice, in that the gp70 is the predominant antigen in the immune complexes deposited in the diseased renal glomeruli.
我们发现，狼疮带试验（LBT）在新西兰黑鼠（NZB）以及新西兰黑鼠与新西兰白（NZW）杂交的（B/W）F1代杂种小鼠的尾部皮肤中常常呈阳性。在雌性NZB、NZW、B/W F1代杂种小鼠以及B/W F1代与NZW回交小鼠中研究了这种阳性反应的遗传发展模式，到12月龄时，其发生率分别为60%、0%、100%和42%。结果发现，B/W F1代杂种小鼠不仅LBT阳性发生率更高，而且其发病时间比亲代NZB小鼠更早。这些发现表明，NZB品系中的一个显性基因座决定了LBT阳性的出现，并且在B/W F1代杂种小鼠中，NZW基因的参与在很大程度上强化了这一性状。连锁研究表明，NZB基因在一定程度上与H-2复合体连锁。此外，在B/W F1代与NZW回交小鼠中，LBT阳性与抗双链DNA抗体的出现以及肾脏疾病之间存在显著关联。因此，有人提出，NZB-LBT基因本身就是该基因，或者在17号染色体上与部分抗DNA抗体及肾脏疾病相关基因紧密连锁。在NZB和B/W F1代杂种小鼠的真皮-表皮交界处缺乏逆转录病毒gp70沉积，因为gp70是沉积在患病肾小球免疫复合物中的主要抗原。
