miRNAs 表达降低作为轴性脊柱关节炎的潜在生物标志物。
Reduced expression of miRNAs as potential biomarkers in axial spondyloarthritis.
机构信息
Mersin University, Faculty of Medicine, Department of Internal Medicine, Division of Rheumatology - Mersin, Turkey.
Mersin University, Faculty of Medicine, Department of Medical Biochemistry - Mersin, Turkey.
出版信息
Rev Assoc Med Bras (1992). 2024 May 3;70(4):e20231521. doi: 10.1590/1806-9282.20231521. eCollection 2024.
OBJECTIVE
This study aimed to investigate the value of miR-29a-3p, miR-27a, miR126-3p, miR-146a-5p, miR-625-3p, miR-130a, miR-32, miR-218, miR-131, and miR5196 in the diagnosis of axial spondyloarthritis and to determine whether there is a difference in miRNA expression levels between radiographic axial spondyloarthritis and non-radiographic axial spondyloarthritis, as well as the relationship between miRNA expression levels, disease activity, and uveitis history.
METHODS
This study included 50 patients with axial spondyloarthritis (25 with radiographic axial spondyloarthritis and 25 with non-radiographic axial spondyloarthritis) and 25 healthy individuals. The fold change of miRNA expression for each miRNA was calculated using the 2-ΔΔCt method.
RESULTS
The expression of all miRNAs except miR-130a was downregulated in axial spondyloarthritis patients (miR-27a: fold regulation: -11.21, p<0.001; miR-29a-3p: fold regulation: -2.63, p<0.001; miR-32: fold regulation: -2.94, p=0.002; miR-126-3p: fold regulation -10.94, p<0.001; miR-132: fold regulation: -2.18, p<0.001; miR-146-5p: fold regulation: -9.78, p<0.001; miR-218: fold regulation: -2.65, p<0.001; miR-625-3p: fold regulation: -2.01, p=0.001; miR-5196-3p: fold regulation: -7.04, p<0.001). The expression levels of these miRNAs did not differ significantly between non-radiographic axial spondyloarthritis and radiographic axial spondyloarthritis patients (p>0.05 for all).
CONCLUSION
Particularly, miR-27a, miR-126-3p, miR-146-5p, and miR-5196-3p were found to be substantially downregulated in both non-radiographic axial spondyloarthritis and radiographic axial spondyloarthritis patients, suggesting their potential as diagnostic biomarkers for axial spondyloarthritis.
目的
本研究旨在探讨 miR-29a-3p、miR-27a、miR126-3p、miR-146a-5p、miR-625-3p、miR-130a、miR-32、miR-218、miR-131 和 miR5196 在轴性脊柱关节炎诊断中的价值，并确定其在放射学轴性脊柱关节炎和非放射学轴性脊柱关节炎患者之间的 miRNA 表达水平是否存在差异，以及 miRNA 表达水平与疾病活动度和葡萄膜炎病史之间的关系。
方法
本研究纳入 50 例轴性脊柱关节炎患者（25 例放射学轴性脊柱关节炎，25 例非放射学轴性脊柱关节炎）和 25 名健康对照者。采用 2-ΔΔCt 法计算每个 miRNA 的 miRNA 表达倍数变化。
结果
除 miR-130a 外，所有 miRNA 的表达在轴性脊柱关节炎患者中均下调（miR-27a：倍数调节：-11.21，p<0.001；miR-29a-3p：倍数调节：-2.63，p<0.001；miR-32：倍数调节：-2.94，p=0.002；miR-126-3p：倍数调节：-10.94，p<0.001；miR-132：倍数调节：-2.18，p<0.001；miR-146-5p：倍数调节：-9.78，p<0.001；miR-218：倍数调节：-2.65，p<0.001；miR-625-3p：倍数调节：-2.01，p=0.001；miR-5196-3p：倍数调节：-7.04，p<0.001）。非放射学轴性脊柱关节炎和放射学轴性脊柱关节炎患者之间这些 miRNA 的表达水平无显著差异（p>0.05）。
结论
特别是 miR-27a、miR-126-3p、miR-146-5p 和 miR-5196-3p 在非放射学轴性脊柱关节炎和放射学轴性脊柱关节炎患者中均明显下调，提示其作为轴性脊柱关节炎诊断生物标志物的潜力。
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