Vadas M A, Clark-Lewis I
Exp Hematol. 1985 Feb;13(2):151-6.
Murine EL-4 thymoma was found to produce a factor that activated antibody-dependent cell-mediated cytotoxicity by human eosinophils and neutrophils. The eosinophil-activating factor had some properties similar to human eosinophil colony-stimulating factor in being heat stable, inactivated by 2-mercaptoethanol, and cochromatographing by gel filtration with a factor that promoted the growth of pure eosinophil colonies from human bone marrow. The neutrophil-activating factor from EL-4 was also heat stable but was not inactivated by 2-mercaptoethanol and was found in two peaks on chromatography, neither having human neutrophil-CSF activity but one cochromatographing with mouse granulocyte-macrophage CSF. EL-4 therefore secretes factors that activate two types of human granulocytes. Several other murine thymomas and macrophage cell lines produced a neutrophil-activating factor, the production of which could not be correlated with that of several other known lymphokines. Murine tumors produce factors that activate human granulocytes.
已发现小鼠EL-4胸腺瘤可产生一种因子，该因子能激活人嗜酸性粒细胞和中性粒细胞的抗体依赖性细胞介导的细胞毒性。嗜酸性粒细胞激活因子具有一些与人类嗜酸性粒细胞集落刺激因子相似的特性，即热稳定、可被2-巯基乙醇灭活，并且通过凝胶过滤与一种能促进人骨髓中纯嗜酸性粒细胞集落生长的因子共层析。来自EL-4的中性粒细胞激活因子同样热稳定，但不被2-巯基乙醇灭活，在层析时出现两个峰，均不具有人类中性粒细胞集落刺激因子活性，但其中一个峰与小鼠粒细胞-巨噬细胞集落刺激因子共层析。因此，EL-4分泌能激活两种人类粒细胞的因子。其他几种小鼠胸腺瘤和巨噬细胞系产生一种中性粒细胞激活因子，其产生与其他几种已知淋巴因子的产生无关。小鼠肿瘤产生能激活人类粒细胞的因子。
