Department of Chemical and Biomolecular Engineering, University of Delaware, 150 Academy Street, Newark, Delaware 19716, United States.
Department of Chemistry, University of Pennsylvania, 231 S. 34th Street, Philadelphia, Pennsylvania 19104, United States.
Biomacromolecules. 2024 Jun 10;25(6):3775-3783. doi: 10.1021/acs.biomac.4c00355. Epub 2024 May 8.
Unlike naturally derived peptides, computationally designed sequences offer programmed self-assembly and charge display. Herein, new tetrameric, coiled coil-forming peptides were computationally designed ranging from 8 to 29 amino acids in length. Experimental investigations revealed that only the sequences having three or more heptads (i.e., 21 or more amino acids) exhibited coiled coil behavior. The shortest stable coiled coil sequence had a melting temperature () of approximately 58 ± 1 °C, making it ideal for thermoreversible assembly over moderate temperatures. Effects of pH and monovalent salt were examined, revealing structural stability over a pH range of 4 to 11 and an enhancement in with the addition of salt. The incorporation of the coiled coil as a hydrogel cross-linker results in a thermally and mechanically reversible hydrogel. A subsequent demonstration of the hydrogel printed through a syringe illustrated one of many potential uses from 3D printing to injectable hydrogel drug delivery.
与天然衍生的肽不同，计算设计的序列提供了可编程的自组装和电荷展示。本文中，设计了新的四聚体、卷曲螺旋形成肽，其长度从 8 到 29 个氨基酸不等。实验研究表明，只有具有三个或更多七肽（即 21 个或更多氨基酸）的序列表现出卷曲螺旋行为。最短的稳定卷曲螺旋序列的熔点（）约为 58±1°C，使其成为中温下热可逆组装的理想选择。考察了 pH 值和单价盐的影响，结果表明，在 pH 值为 4 到 11 的范围内具有结构稳定性，并且随着盐的加入而增强。将卷曲螺旋用作水凝胶交联剂导致水凝胶具有热和机械可逆性。通过注射器进行水凝胶打印的后续演示说明了 3D 打印在可注射水凝胶药物输送等许多潜在用途中的一个。
