Kumar A, Kiefer J, Schneider E, Crompton N E
Int J Radiat Biol Relat Stud Phys Chem Med. 1985 Jan;47(1):103-12. doi: 10.1080/09553008514550121.
X-ray induced potentially lethal damage and its inhibition by the aromatic amide 3-aminobenzamide have been investigated in Chinese hamster V79 cells. 3-Aminobenzamide (3-AB) is a known inhibitor of polyadenosine diphosphoribose synthetase. With increasing concentrations of 3-AB an increasing inhibition of PLD repair was observed. Little inhibition of PLD repair was seen when 3-AB was added 3 h following irradiation. Utilizing the 6-thioguanine mutation assay, the effect of poly(ADP-R) synthetase inhibition under conditions of PLD repair upon mutation frequency were also studied. A large increase in mutation frequency following 24 h post-irradiation recovery in the presence of 3-AB was seen. These results favour a possible role of 3-AB in preventing repair by facilitating early damage fixation before repair can occur, simultaneously reducing G2-arrest.
在中国仓鼠V79细胞中，研究了X射线诱导的潜在致死性损伤及其被芳香酰胺3 - 氨基苯甲酰胺抑制的情况。3 - 氨基苯甲酰胺（3 - AB）是一种已知的聚腺苷二磷酸核糖合成酶抑制剂。随着3 - AB浓度的增加，观察到对潜在致死性损伤修复的抑制作用增强。当在照射后3小时添加3 - AB时，对潜在致死性损伤修复的抑制作用较小。利用6 - 硫鸟嘌呤突变试验，还研究了在潜在致死性损伤修复条件下，聚（ADP - R）合成酶抑制对突变频率的影响。在存在3 - AB的情况下，照射后24小时恢复时，观察到突变频率大幅增加。这些结果表明，3 - AB可能通过在修复发生之前促进早期损伤固定来防止修复，同时减少G2期阻滞。
