Senior Professor, Medicine, Sardar Patel Medical College, Bikaner, Rajasthan, India.
Senior Resident, Radiodiagnosis, Sardar Patel Medical College, Bikaner, Rajasthan, India.
J Assoc Physicians India. 2023 Nov;71(11):50-52. doi: 10.59556/japi.71.0396.
: Micro ribonucleic acids (miRNAs) are noncoding RNAs, recently implicated as potential biomarkers or therapeutic targets for autoimmune diseases such as rheumatoid arthritis (RA). The aim of this study is to assess the role of miRNA Let-7 in the plasma of RA. : Trained medical staff already enrolled for the study collected blood samples from healthy controls ( = 42) and RA patients ( = 44). In the laboratory, these samples were centrifuged at 2000 rpm for 8 minutes to separate serum from the sample, which was then transferred to a plain vial. Until transport to the genetic lab, samples were stored at -20°C Deoxyribonucleic acid (DNA) was isolated using a standard protocol cohort of controls and patient blood samples. Quantification of DNA was conducted using ultraviolet (UV) spectroscopy, and DNA quality was assessed on 0.8% agarose gel. A comparison of genotype frequencies in the different study groups was performed using the Chi-squared test, while a comparison of allelic frequencies was conducted using Fisher's exact test. : Variations of alleles, such as 16539423 (G>T), 16539433 (T>G), and 16539629 (A>T) were found only in RA patients. On the other hand, 16539617 (T>A), 16539622 (G>T), and 16539624 (T>C) were found only in control cases. Five sequences (three RA variants and two control variants) with minimal alignment were compared to the wild-type sequence. We found that the sequence modification of pre-miRNA 16539623 del G was significantly higher and had a risk allele in the study group [odds ratio (OR) = 3.29]. : Rheumatoid arthritis (RA) is an autoimmune disorder that presents with a variety of clinical manifestations. Genetic factors possibly account for about 60% of disease susceptibility and expression, thus playing a very important role in disease pathogenesis. : Gauri LA, Liyakat N, Dadhich D, Role of miRNA Let-7 in Plasma of Rheumatoid Arthritis. J Assoc Physicians India 2023;71(11):50-52.
: 微小核糖核酸（miRNAs）是非编码 RNA，最近被认为是类风湿关节炎（RA）等自身免疫性疾病的潜在生物标志物或治疗靶点。本研究旨在评估 miRNA Let-7 在 RA 血浆中的作用。: 经过培训的医务人员已经为该研究招募了健康对照组（=42）和 RA 患者（=44）的血液样本。在实验室中，将这些样本以 2000rpm 的速度离心 8 分钟，以将血清与样本分离，然后将其转移到普通小瓶中。在运送到遗传实验室之前，将样本储存在-20°C 下。使用标准协议从对照和患者的血液样本中分离 DNA。使用紫外线（UV）光谱法进行 DNA 定量，并在 0.8%琼脂糖凝胶上评估 DNA 质量。使用卡方检验比较不同研究组的基因型频率，使用 Fisher 确切检验比较等位基因频率。: 仅在 RA 患者中发现了等位基因变异，如 16539423（G>T）、16539433（T>G）和 16539629（A>T）。另一方面，仅在对照病例中发现了 16539617（T>A）、16539622（G>T）和 16539624（T>C）。将五个具有最小比对的序列（三个 RA 变体和两个对照变体）与野生型序列进行比较。我们发现，研究组中 miRNA 16539623delG 的序列修饰显著更高，并且具有风险等位基因[比值比（OR）=3.29]。: 类风湿关节炎（RA）是一种自身免疫性疾病，具有多种临床表现。遗传因素可能占疾病易感性和表达的 60%左右，因此在疾病发病机制中起着非常重要的作用。: Gauri LA、Liyakat N、Dadhich D，miRNA Let-7 在类风湿关节炎血浆中的作用。J 印度医师协会 2023 年；71（11）：50-52。
