Mao Yifan, Lv Weiwei, Huang Weiwei, Yuan Quan, Yang Hang, Zhou Wenzong, Li Mingyou
Key Laboratory of Integrated Rice-Fish Farming, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, China.
Eco-Environmental Protection Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, China.
Front Physiol. 2024 Apr 24;15:1397818. doi: 10.3389/fphys.2024.1397818. eCollection 2024.
To investigate the impact of the effect of high temperature stimulation on larvae after a certain period of time, five experimental groups were established at different temperatures. Then, the under high temperature stress was fed at 30°C for 70 days. After that, the growth index of the was counted and analyzed. In terms of growth index, high temperature stress had significant effects on FCR, FBW, WGR, and SGR of ( 0.05). The SR increased after being stimulated by temperature ( 0.1). The study revealed that liver cells of were harmed by elevated temperatures of 36°C and 38°C. In the experimental group, the activities of digestive enzymes changed in the same trend, reaching the highest point in the 32°C group and then decreasing, and the AMS activity in the 38°C group was significantly different from that in the 30°C group ( < 0.05). The activities of antioxidase in liver reached the highest at 34°C, which was significantly different from those at 30°C ( < 0.05). In addition, the expression levels of , , , and other genes increased in the experimental group, reaching the highest point at 34°C, and the expression level of the gene reached the highest point at 32°C, which was significantly different from that at 30°C ( < 0.05). However, the expression level of the gene decreased in the experimental group and reached its lowest point at 34°C ( < 0.05). The expression level of the gene increased with the highest temperature stimulus and reached its highest point at 38°C ( < 0.05). In the α diversity index of intestinal microorganisms in the experimental group, the observed species, Shannon, and Chao1 indexes in the 34°C group were the highest ( < 0.05), and β diversity analysis revealed that the intestinal microbial community in the experimental group was separated after high temperature stimulation. At the phylum level, the three dominant flora are , , and . and abundance increased at the genus level, but and abundance decreased. To sum up, appropriate high-temperature stress can enhance the immunity and adaptability of . These results show that the high temperature stimulation of 32°C-34°C is beneficial to the industrial culture of .
为研究高温刺激在一定时间后对幼虫的影响,设置了五个不同温度的实验组。然后,将处于高温胁迫下的幼虫在30℃饲养70天。之后,对幼虫的生长指标进行计数和分析。在生长指标方面,高温胁迫对幼虫的饲料系数(FCR)、终末体重(FBW)、增重率(WGR)和特定生长率(SGR)有显著影响(P<0.05)。存活率(SR)在温度刺激后升高(P>0.1)。研究表明,36℃和38℃的高温会损害幼虫的肝细胞。在实验组中,消化酶活性呈相同趋势变化,在32℃组达到最高点,然后下降,38℃组的淀粉酶(AMS)活性与30℃组有显著差异(P<0.05)。肝脏中抗氧化酶活性在34℃时达到最高,与30℃时的活性有显著差异(P<0.05)。此外,实验组中热休克蛋白(HSP)、金属硫蛋白(MT)、过氧化氢酶(CAT)等基因的表达水平升高,在34℃时达到最高点,热休克蛋白70(HSP70)基因的表达水平在32℃时达到最高点,与30℃时的表达水平有显著差异(P<0.05)。然而,实验组中热休克蛋白90(HSP90)基因的表达水平下降,在34℃时达到最低点(P<0.05)。热休克蛋白10(HSP10)基因的表达水平随着最高温度刺激而升高,在38℃时达到最高点(P<0.05)。在实验组肠道微生物的α多样性指数中,34℃组的观测物种数、香农指数和 Chao1指数最高(P<0.05),β多样性分析表明,实验组的肠道微生物群落在高温刺激后发生了分离。在门水平上,三个优势菌群为厚壁菌门(Firmicutes)、拟杆菌门(Bacteroidetes)和变形菌门(Proteobacteria)。在属水平上,芽孢杆菌属(Bacillus)和双歧杆菌属(Bifidobacterium)丰度增加,但乳杆菌属(Lactobacillus)和肠球菌属(Enterococcus)丰度下降。综上所述,适当的高温胁迫可以增强幼虫的免疫力和适应性。这些结果表明,32℃-34℃的高温刺激有利于幼虫的工业化养殖。