石蜡组织中的三种微小RNA组合可作为预测肾细胞癌预后的工具。
A three miRNAs panel in paraffin tissue serves as tool for predicting prognosis of renal cell carcinoma.
作者信息
Chen Wenkang, Wang Wuping, Zhao Zhengping, Wen Zhenyu, Li Yingqi, Ge Zhenjian, Lai Yongqing, Ni Liangchao
机构信息
Guangdong and Shenzhen Key Laboratory of Reproductive Medicine and Genetics, Department of Urology, Peking University Shenzhen Hospital, Shenzhen, China.
Shantou University Medical College, Shantou, Guangdong, China.
出版信息
Front Oncol. 2024 Apr 24;14:1391844. doi: 10.3389/fonc.2024.1391844. eCollection 2024.
BACKGROUND
Renal cell carcinoma (RCC) stands as the most prevalent form of urogenital cancer. However, there is currently no universally accepted method for predicting the prognosis of RCC. MiRNA holds great potential as a prognostic biomarker for RCC.
METHODS
A total of 100 cases with complete paraffin specimens and over 5-year follow-up data meeting the requirements were collected. Utilizing the clinical information and follow-up data of the specimens, an information model was developed. The expression levels of eight microRNAs were identified using RT-qPCR. Finally, determine and analyze the clinical application value of these microRNAs as prognostic markers for RCC.
RESULTS
Significant differences were observed in the expression of two types of miRNAs (miR-378a-5p, miR-23a-5p) in RCC tissue, and three types of miRNAs (miR-378a-5p, miR-642a-5p, miR-23a-5p) were found to be linked to the prognosis of RCC. Establish biomarker combinations of miR-378a-5p, miR-642a-5p, and miR-23a-5p to evaluate RCC prognosis.
CONCLUSION
The combination of three microRNA groups (miR-378a-5p, miR-642a-5p, and miR-23a-5p) identified in paraffin section specimens of RCC in this study holds significant potential as biomarkers for assessing RCC prognosis.
背景
肾细胞癌（RCC）是泌尿生殖系统癌症中最常见的形式。然而，目前尚无普遍接受的预测RCC预后的方法。微小RNA（miRNA）作为RCC的预后生物标志物具有巨大潜力。
方法
收集了100例符合要求的具有完整石蜡标本且随访数据超过5年的病例。利用标本的临床信息和随访数据建立了一个信息模型。采用逆转录定量聚合酶链反应（RT-qPCR）鉴定了8种微小RNA的表达水平。最后，确定并分析这些微小RNA作为RCC预后标志物的临床应用价值。
结果
在RCC组织中观察到两种微小RNA（miR-378a-5p、miR-23a-5p）的表达存在显著差异，并且发现三种微小RNA（miR-378a-5p、miR-642a-5p、miR-23a-5p）与RCC的预后相关。建立miR-378a-5p、miR-642a-5p和miR-23a-5p的生物标志物组合以评估RCC预后。
结论
本研究在RCC石蜡切片标本中鉴定出的三组微小RNA（miR-378a-5p、miR-642a-5p和miR-23a-5p）组合作为评估RCC预后的生物标志物具有巨大潜力。
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