Danese Elisa, Padoan Andrea, Negrini Davide, Paviati Elisa, De Pastena Matteo, Esposito Alessandro, Lippi Giuseppe, Montagnana Martina
Section of Clinical Biochemistry, Department of Engineering for Innovation Medicine, 19051 University of Verona , Verona, Italy.
Department of Medicine - DIMED, University of Padova, Padova, Italy.
Clin Chem Lab Med. 2024 May 9;62(11):2287-2293. doi: 10.1515/cclm-2024-0196. Print 2024 Oct 28.
There is a growing interest in the relevance of salivary cortisol and cortisone concentrations in stress-related research. To correctly attribute the magnitude of salivary cortisol and cortisone variation as an effect of a stressful event, a coherent understanding of the day-to-day intra-individual and inter-individual variability across the diurnal cycle of the two steroids is required. However, such information is currently lacking.
This study aimed to overcome these existing limitations by performing an investigation of the biological variation (BV) of salivary cortisol and cortisone within one day and between five days using an LC-MS/MS method. Saliva samples were collected from 20 healthy volunteers immediately after waking up, at 8:00, 12:00, 15:00, 19:00 and 23:00 on each day over five days. All samples were analyzed in duplicate in one run. Nested ANOVA was used to calculate the sums of squares for analytical and biological components of variation.
The within-subject BV of salivary cortisol and cortisone (CV) ranged from a minimum of 29.3 and 19.0 % to a maximum of 56.5 and 49.1 %, respectively, while the between-subject biological variation (CV) ranged from 29.7 and 29.0 % to 51.6 and 43.6 %. The reference change values (RCVs) ranged from 96 to 245 % for cortisol and from 55 to 194 % for cortisone. A medium index of individuality was observed for both compounds at all time points.
This study provides updated BV estimates and RCVs for different times of day that can be used to assess the magnitude of change in biomarkers in future stress-related research.
在应激相关研究中，唾液皮质醇和可的松浓度的相关性日益受到关注。为了正确将唾液皮质醇和可的松变化的幅度归因于应激事件的影响，需要对这两种类固醇在昼夜周期内的个体内和个体间每日变异性有连贯的理解。然而，目前缺乏此类信息。
本研究旨在通过使用液相色谱-串联质谱法（LC-MS/MS）对一天内和五天之间唾液皮质醇和可的松的生物学变异（BV）进行调查，以克服这些现有局限性。在五天内，每天早上醒来后以及8:00、12:00、15:00、19:00和23:00从20名健康志愿者中采集唾液样本。所有样本均在一次分析中进行双份测定。采用嵌套方差分析来计算变异的分析和生物学成分的平方和。
唾液皮质醇和可的松的受试者内BV（CV）分别从最低的29.3%和19.0%到最高的56.5%和49.1%不等，而受试者间生物学变异（CV）分别从29.7%和29.0%到51.6%和43.6%不等。皮质醇的参考变化值（RCV）范围为96%至245%，可的松的参考变化值范围为55%至194%。在所有时间点，两种化合物均观察到中等个体指数。
本研究提供了一天中不同时间的更新BV估计值和RCV，可用于评估未来应激相关研究中生物标志物变化的幅度。
