Department of Chemistry, National Institute of Technology Rourkela, Odisha 769008, India.
Department of Life Science, National Institute of Technology Rourkela, Odisha 769008, India.
Anal Chem. 2024 May 28;96(21):8630-8640. doi: 10.1021/acs.analchem.4c00708. Epub 2024 May 9.
Development of reliable methods for the detection of potential biomarkers is of the utmost importance for an early diagnosis of critical diseases and disorders. In this study, a novel lanthanide-functionalized carbon dot-based fluorescent probe Zn-CD@Eu is reported for the ratiometric detection of dipicolinic acid (DPA) and uric acid (UA). The Zn-CD@Eu nanoprobe was obtained from a simple room-temperature reaction of zinc-doped carbon dots (Zn-CD) and the EDTA-Eu lanthanide complex. Under optimal conditions, a good linear response was obtained for DPA in two concentration ranges of 0-55 and 55-100 μM with a limit of detection of 0.53 and 2.2 μM respectively, which is significantly below the infectious dosage of anthrax (∼55 μM). Furthermore, the Zn-CD@Eu/DPA system was employed for the detection of UA with a detection limit of 0.36 μM in the linear range of 0-100 μM. The fluorescent probe was successfully implemented for determining DPA and UA in human blood serum, sweat, and natural water bodies with considerable recovery rates. In addition, the potential of the nanoprobe for ex vivo visualization of UA was demonstrated in fruit fly () as a model organism.
开发可靠的方法来检测潜在的生物标志物对于早期诊断严重疾病和障碍至关重要。在本研究中，报道了一种新型镧系元素功能化碳点荧光探针 Zn-CD@Eu，用于检测二吡咯羧酸（DPA）和尿酸（UA）的比率。Zn-CD@Eu 纳米探针是通过锌掺杂碳点（Zn-CD）和 EDTA-Eu 镧系配合物的简单室温反应获得的。在最佳条件下，DPA 在两个浓度范围内（0-55 和 55-100 μM）的线性响应良好，检出限分别为 0.53 和 2.2 μM，远低于炭疽的感染剂量（约 55 μM）。此外，Zn-CD@Eu/DPA 体系可用于检测 UA，在 0-100 μM 的线性范围内检测限为 0.36 μM。该荧光探针成功用于测定人血清、汗液和天然水体中的 DPA 和 UA，回收率相当高。此外，还通过果蝇（）作为模式生物证明了纳米探针用于 UA 体外可视化的潜力。
