Wang Yingjie, Li Xiang, Lv Xinru, Li Yi, An Qing, Xiu Yang, Lv Xiangtong, Song Haozhuo, Zhou Changyu, Xu Qiushi, Qiao Dan, Yang Siyuan, Chai Hongliang, Zeng Xiangwei
College of Wildlife and Protected Area, Northeast Forestry University, Harbin, 150040, China.
Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu, 610064, China.
Virus Genes. 2024 Jun;60(3):320-324. doi: 10.1007/s11262-024-02068-5. Epub 2024 May 9.
H6 avian influenza virus is widely prevalent in wild birds and poultry and has caused human infection in 2013 in Taiwan, China. During our active influenza surveillance program in wild waterfowl at Poyang Lake, Jiangxi Province, an H6N2 AIV was isolated and named A/bean goose/JiangXi/452-4/2013(H6N2). The isolate was characterized as a typical low pathogenic avian influenza virus (LPAIV) due to the presence of the amino acid sequence PQIETR↓GLFGAI at the cleavage site of the hemagglutinin (HA) protein. The genetic evolution analysis revealed that the NA gene of the isolate originated from North America and exhibited the highest nucleotide identity (99.29%) with a virus recovered from wild bird samples in North America, specifically A/bufflehead/California/4935/2012(H11N2). Additionally, while the HA and PB1 genes belonged to the Eurasian lineage, they displayed frequent genetic interactions with the North American lineage. The remaining genes showed close genetic relationships with Eurasian viruses. The H6N2 isolate possessed a complex genome, indicating it is a multi-gene recombinant virus with genetic material from both Eurasian and North American lineages.
H6禽流感病毒在野生鸟类和家禽中广泛流行，并于2013年在中国台湾地区导致人类感染。在我们对江西省鄱阳湖野生水禽开展的主动流感监测项目中，分离出一株H6N2禽流感病毒，并命名为A/豆雁/江西/452-4/2013(H6N2)。由于血凝素(HA)蛋白裂解位点存在氨基酸序列PQIETR↓GLFGAI，该分离株被鉴定为典型的低致病性禽流感病毒(LPAIV)。遗传进化分析显示，该分离株的NA基因起源于北美，与从北美野生鸟类样本中分离出的一株病毒（具体为A/巨头鹊鸭/加利福尼亚/4935/2012(H11N2)）具有最高的核苷酸同一性(99.29%)。此外，虽然HA和PB1基因属于欧亚谱系，但它们与北美谱系表现出频繁的基因相互作用。其余基因与欧亚病毒显示出密切的遗传关系。该H6N2分离株具有复杂的基因组，表明它是一种具有来自欧亚和北美谱系遗传物质的多基因重组病毒。
