Suzuki S
Int J Radiat Biol Relat Stud Phys Chem Med. 1985 Mar;47(3):291-7. doi: 10.1080/09553008514550431.
When exposed to either u.v. radiation or 60Co gamma-rays, the thermophilic bacterium, Thermus thermophilus HB-8, which can grow at 49-85 degrees C, lost its ability to take up extracellular K+ in a dose-dependent manner. However, the loss was reduced by incubation at 37 degrees C after exposure to u.v. radiation or gamma-rays. Cell survival after exposure to 60Co gamma-rays, as measured by colony formation, was increased by incubation at 37 degrees C after exposure, whereas cell survival after u.v. radiation was not. These results, therefore, indicate that the loss of ability of cells to take up K+ after u.v. radiation was not due to cell death but some damage to the membrane itself, and that the membrane damage can be repaired. Lipid peroxidation is not responsible for the membrane damage, because HB-8 cells do not contain unsaturated fatty acids in their membranes.
当嗜热栖热菌HB - 8暴露于紫外线辐射或60Coγ射线时，这种能在49 - 85摄氏度生长的嗜热细菌，会以剂量依赖的方式丧失摄取胞外钾离子的能力。然而，在暴露于紫外线辐射或γ射线后于37摄氏度培养，这种能力丧失会有所减少。通过菌落形成来衡量，暴露于60Coγ射线后的细胞存活率，在暴露后于37摄氏度培养时会增加，而紫外线辐射后的细胞存活率则不会。因此，这些结果表明，紫外线辐射后细胞摄取钾离子能力的丧失并非由于细胞死亡，而是膜本身受到了某种损伤，并且这种膜损伤是可以修复的。脂质过氧化并非膜损伤的原因，因为HB - 8细胞的膜中不含有不饱和脂肪酸。
