Microbiology and Molecular Diagnostics Lab, Infection Control, Max Super Speciality Hospital, Saket, New Delhi, India.
HaystackAnalytics Pvt. Ltd., Society for Innovation & Entrepreneurship (SINE), Kanwal Rekhi Building, Indian Institute of Technology Bombay, Powai, Mumbai, 400076, India.
Indian J Med Microbiol. 2024 May-Jun;49:100608. doi: 10.1016/j.ijmmb.2024.100608. Epub 2024 May 13.
Timely diagnosis and treatment of sepsis is a major challenge faced by critical care specialists around the world. The traditional blood culture methods have a significant turnaround time which delays targeted therapy leading to poor prognosis. In the current study, we highlight the clinical utility of a genomics solution for diagnosis and management of bloodstream infections by combining the real-time DNA sequencing of Oxford Nanopore Technology with an automated genomic data analysis software. We identify a carbapenem-resistant Klebsiella pneumoniae directly from a blood sample in <24 hours and thereby prove the effectiveness of the test in early diagnosis of sepsis.
及时诊断和治疗脓毒症是全球重症监护专家面临的主要挑战。传统的血培养方法有显著的周转时间，这会延迟靶向治疗，导致预后不良。在当前的研究中，我们通过将牛津纳米孔技术的实时 DNA 测序与自动化基因组数据分析软件相结合，强调了基因组学解决方案在诊断和管理血流感染方面的临床应用。我们在不到 24 小时的时间内直接从血样中鉴定出耐碳青霉烯类肺炎克雷伯菌，从而证明了该检测在脓毒症早期诊断中的有效性。
