ST37 肺炎克雷伯菌：新生儿抗菌治疗期间体内碳青霉烯类耐药性的发展
ST37 Klebsiella pneumoniae: development of carbapenem resistance in vivo during antimicrobial therapy in neonates.
作者信息
Li Pengling, Wang Min, Li Xianping, Hu Feihu, Yang Min, Xie Yixin, Cao Wei, Xia Xiaomeng, Zheng Rong, Tian Jingjing, Zhang Kan, Chen Fang, Tang Aiguo
机构信息
Department of Laboratory Medicine, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, China.
Department of Psychiatry, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, China.
出版信息
Future Microbiol. 2017 Aug;12:891-904. doi: 10.2217/fmb-2016-0165. Epub 2017 Jul 12.
AIM
To investigate the mechanism leading to in vivo carbapenem resistance development in Klebsiella pneumoniae.
METHODS
Carbapenemase was detected using the modified carbapenem inactivation method. β-lactamases resistant genes were identified by PCR and sequencing. Clonal relatedness was evaluated by random amplified polymorphic DNA and multiple locus sequence typing. The relationship between sequence typing and resistant genes was analyzed by using the chi-squared test.
RESULTS
All ST37 carbapenem-resistant isolates were bla positive and all ST37 carbapenem-sensitive isolates were bla negative at Stage I. A significant relationship between carbapenem resistance and bla was observed. The bla -positive rate was significantly higher in ST37 K. pneumoniae than others.
CONCLUSION
This is the first study about the development of carbapenem resistance in vivo potentially mediated by bla in ST37 K. pneumoniae among neonates.
目的
研究肺炎克雷伯菌体内碳青霉烯类耐药性产生的机制。
方法
采用改良碳青霉烯灭活法检测碳青霉烯酶。通过聚合酶链反应（PCR）和测序鉴定β-内酰胺酶耐药基因。采用随机扩增多态性DNA和多位点序列分型评估克隆相关性。使用卡方检验分析序列分型与耐药基因之间的关系。
结果
在第一阶段，所有ST37碳青霉烯耐药菌株bla均为阳性，所有ST37碳青霉烯敏感菌株bla均为阴性。观察到碳青霉烯耐药性与bla之间存在显著关系。ST37肺炎克雷伯菌的bla阳性率显著高于其他菌株。
结论
这是关于新生儿中ST37肺炎克雷伯菌体内bla潜在介导的碳青霉烯耐药性发展的首次研究。
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