State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; CAS Center for Excellence in Biotic Interactions, University of Chinese Academy of Sciences, Beijing 100049, China.
State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; CAS Center for Excellence in Biotic Interactions, University of Chinese Academy of Sciences, Beijing 100049, China.
Comp Biochem Physiol A Mol Integr Physiol. 2024 Sep;295:111655. doi: 10.1016/j.cbpa.2024.111655. Epub 2024 May 7.
Ambient temperatures have great impacts on thermoregulation of small mammals. Brown adipose tissue (BAT), an obligative thermogenic tissue for small mammals, is localized not only in the interscapular depot (iBAT), but also in supraclavicular, infra/subscapular, cervical, paravertebral, and periaortic depots. The iBAT is known for its cold-induced thermogenesis, however, less has been paid attention to the function of BAT at other sites. Here, we investigated the function of BAT at different sites of the body during cold acclimation in a small rodent species. As expected, Brandt's voles (Lasiopodomys brandtii) consumed more food and reduced the body mass gain when they were exposed to cold. The voles increased resting metabolic rate and maintained a relatively lower body temperature in the cold (36.5 ± 0.27 °C) compared to those in the warm condition (37.1 ± 0.36 °C). During cold acclimation, the uncoupling protein 1 (UCP1) increased in aBAT (axillary), cBAT (anterior cervical), iBAT (interscapular), nBAT (supraclavicular), and sBAT (suprascapular). The levels of proliferating cell nuclear antigen (PCNA), a marker for cell proliferation, were higher in cBAT and iBAT in the cold than in the warm group. The pAMPK/AMPK and pCREB/CREB were increased in cBAT and iBAT during cold acclimation, respectively. These data indicate that these different sites of BAT play the cold-induced thermogenic function for small mammals.
环境温度对小型哺乳动物的体温调节有很大影响。棕色脂肪组织（BAT）是小型哺乳动物必需的产热组织，不仅存在于肩胛间（iBAT），还存在于锁骨上、锁骨下、颈部、椎旁和主动脉周围。iBAT 以其冷诱导产热而闻名，然而，其他部位的 BAT 功能却较少受到关注。在这里，我们研究了小型啮齿动物在冷适应过程中身体不同部位的 BAT 功能。正如预期的那样，布氏田鼠（Lasiopodomys brandtii）在暴露于寒冷环境时会消耗更多的食物，并减少体重增加。与温暖条件（37.1 ± 0.36°C）相比，布氏田鼠在寒冷（36.5 ± 0.27°C）中增加了静息代谢率并保持相对较低的体温。在冷适应过程中，解偶联蛋白 1（UCP1）在 aBAT（腋窝）、cBAT（前颈）、iBAT（肩胛间）、nBAT（锁骨上）和 sBAT（肩胛上）中增加。增殖细胞核抗原（PCNA）的水平，细胞增殖的标志物，在冷组中的 cBAT 和 iBAT 中高于暖组。冷适应过程中，cBAT 和 iBAT 中的 pAMPK/AMPK 和 pCREB/CREB 增加。这些数据表明，这些 BAT 的不同部位在小型哺乳动物中发挥冷诱导产热功能。
